Yeast Two-Hybrid Screening of Photoswitchable Protein-Protein Interaction Libraries.

Woloschuk, Ryan M; Reed, P Maximilian M; McDonald, Sherin; Uppalapati, Maruti; Woolley, G Andrew

Woloschuk, Ryan M; Reed, P Maximilian M; McDonald, Sherin; Uppalapati, Maruti; Woolley, G Andrew.

Affiliation

Woloschuk RM; Department of Chemistry, University of Toronto, 80 St. George St, Toronto, Canada, M5S 3H6.
Reed PMM; Department of Chemistry, University of Toronto, 80 St. George St, Toronto, Canada, M5S 3H6.
McDonald S; Department of Pathology and Laboratory Medicine, University of Saskatchewan, 107 Wiggins Rd, Saskatoon, SK, Canada, S7N 5E5.
Uppalapati M; Department of Pathology and Laboratory Medicine, University of Saskatchewan, 107 Wiggins Rd, Saskatoon, SK, Canada, S7N 5E5.
Woolley GA; Department of Chemistry, University of Toronto, 80 St. George St, Toronto, Canada, M5S 3H6. Electronic address: andrew.woolley@utoronto.ca.

J Mol Biol ; 432(10): 3113-3126, 2020 05 01.

Article in En | MEDLINE | ID: mdl-32198111

Subject(s)

Bacterial Proteins/metabolism; Photoreceptors, Microbial/metabolism; Protein Interaction Mapping/methods; Proteins/metabolism; Animals; Bacterial Proteins/chemistry; Bacterial Proteins/genetics; Feasibility Studies; Gene Library; Humans; Models, Molecular; Optogenetics; Photoreceptors, Microbial/chemistry; Photoreceptors, Microbial/genetics; Protein Binding; Proteins/genetics; Two-Hybrid System Techniques

Key words

optogenetics; photo-control; photoactive yellow protein; yeast two-hybrid

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Bacterial Proteins / Proteins / Photoreceptors, Microbial / Protein Interaction Mapping Type of study: Diagnostic_studies / Screening_studies Limits: Animals / Humans Language: En Journal: J Mol Biol Year: 2020 Document type: Article Country of publication: Netherlands

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