Polymorphism of glutathione S-transferase in the population of Polish patients with carcinoma of the prostate.
Environ Sci Pollut Res Int
; 27(16): 19375-19382, 2020 Jun.
Article
in En
| MEDLINE
| ID: mdl-32212077
ABSTRACT
The aim of the study was frequency analysis of GSTM1, GSTT1, and GSTP1 polymorphisms of glutathione S-transferase in the group of patients with prostate cancer and in a control group of healthy individuals. Genomic DNA was isolated; molecular analysis of glutathione S-transferase M1 and T2 polymorphisms was performed using multiplex PCR and RFLP methods. The products of the PCR reaction were then visualized in agarose gel, and a statistical analysis of the results was performed. No statistically significant differences were found in the frequency of glutathione S-transferase polymorphisms between 66 patients with prostate cancer and the control group (64 healthy volunteers). The GSTM1 gene deletion was found in ca. 47% of patients with prostate cancer and in ca. 55% of the controls. The GSTT1 deletion was found in approximately 17% of patients and 14% of the controls. The distribution of GSTP1 Ile/Ile, Ile/Val, and Val/Val polymorphisms was ca. 51.5%, 39%, and 9% in the group of patients and 61%, 34%, and 5% in the control group, respectively. The results indicate that there is no relationship between glutathione S-transferase polymorphisms and prostate cancer in the study group, which is a novelty when compared with the previous work on the role of these genetic variants in the etiology of cancer.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Glutathione S-Transferase pi
/
Glutathione Transferase
Type of study:
Etiology_studies
/
Observational_studies
/
Risk_factors_studies
Limits:
Humans
/
Male
Country/Region as subject:
Europa
Language:
En
Journal:
Environ Sci Pollut Res Int
Journal subject:
SAUDE AMBIENTAL
/
TOXICOLOGIA
Year:
2020
Document type:
Article
Affiliation country:
Poland