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Homeobox C8 inhibited the osteo-/dentinogenic differentiation and migration ability of stem cells of the apical papilla via activating KDM1A.
Yang, Haoqing; Liang, Yuncun; Cao, Yangyang; Cao, Yu; Fan, Zhipeng.
Affiliation
  • Yang H; Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Beijing Stomatology Hospital, Capital Medical University, Beijing, China.
  • Liang Y; Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Beijing Stomatology Hospital, Capital Medical University, Beijing, China.
  • Cao Y; Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Beijing Stomatology Hospital, Capital Medical University, Beijing, China.
  • Cao Y; Department of General Dentistry, School of Stomatology, Beijing Stomatology Hospital, Capital Medical University, Beijing, China.
  • Fan Z; Beijing Key Laboratory of Tooth Regeneration and Function Reconstruction, School of Stomatology, Beijing Stomatology Hospital, Capital Medical University, Beijing, China.
J Cell Physiol ; 235(11): 8432-8445, 2020 11.
Article in En | MEDLINE | ID: mdl-32246725
Enhancing the functions of mesenchymal stem cells (MSCs) is considered a potential approach for promoting tissue regeneration. In the present study, we investigate the role of HOXC8 in regulating differentiation and migration by using stem cells of the apical papilla (SCAPs). Our results showed that overexpression of HOXC8 suppressed the osteo-/dentinogenic differentiation, as detected by measuring alkaline phosphatase activity, in vitro mineralization, and the expressions of dentin sialophosphoprotein, dentin matrix acidic phosphoprotein 1, bone sialoprotein, runt-related transcription factor 2, and osterix in SCAPs, and inhibited in vivo osteo-/dentinogenesis of SCAPs. In addition, knockdown of HOXC8 promoted the osteo-/dentinogenic differentiation potentials of SCAPs. Mechanically, HOXC8 enhanced KDM1A transcription by directly binding to its promoter. HOXC8 and KDM1A also inhibited the migration and chemotaxis abilities of SCAPs. To sum up, HOXC8 negatively regulated the osteo-/dentinogenic differentiation and migration abilities of SCAPs by directly enhancing KDM1A transcription and indicated that HOXC8 and KDM1A could serve as potential targets for enhancing dental MSC mediated tissue regeneration.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Stem Cells / Cell Differentiation / Homeodomain Proteins / Histone Demethylases Limits: Humans Language: En Journal: J Cell Physiol Year: 2020 Document type: Article Affiliation country: China Country of publication: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Stem Cells / Cell Differentiation / Homeodomain Proteins / Histone Demethylases Limits: Humans Language: En Journal: J Cell Physiol Year: 2020 Document type: Article Affiliation country: China Country of publication: United States