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Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson's Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation.
Fan, Ying; Tonelli, Francesca; Padmanabhan, Shalini; Baptista, Marco A S; Riley, Lindsey; Smith, Danielle; Marras, Connie; Howden, Andrew; Alessi, Dario R; Sammler, Esther.
Affiliation
  • Fan Y; MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee.
  • Tonelli F; MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee.
  • Padmanabhan S; The Michael J. Fox Foundation for Parkinson's Research.
  • Baptista MAS; The Michael J. Fox Foundation for Parkinson's Research.
  • Riley L; The Michael J. Fox Foundation for Parkinson's Research.
  • Smith D; Department of Medical and Molecular Genetics, Indiana University School of Medicine.
  • Marras C; Morton and Gloria Shulman Movement Disorders Centre and the Edmond J. Safra Program in Parkinson's Disease, Toronto Western Hospital, University of Toronto.
  • Howden A; Division of Cell Signalling and Immunology, School of Life Sciences, University of Dundee.
  • Alessi DR; MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee.
  • Sammler E; MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee; Division of Molecular and Clinical Medicine, School of Medicine, Ninewells Hospital and Medical School, University of Dundee; e.m.sammler@dundee.ac.uk.
J Vis Exp ; (157)2020 03 21.
Article in En | MEDLINE | ID: mdl-32250352
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Parkinson Disease / Rab GTP-Binding Proteins / Leucine-Rich Repeat Serine-Threonine Protein Kinase-2 / Neutrophils Type of study: Risk_factors_studies Limits: Humans Language: En Journal: J Vis Exp Year: 2020 Document type: Article Country of publication: United States
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Parkinson Disease / Rab GTP-Binding Proteins / Leucine-Rich Repeat Serine-Threonine Protein Kinase-2 / Neutrophils Type of study: Risk_factors_studies Limits: Humans Language: En Journal: J Vis Exp Year: 2020 Document type: Article Country of publication: United States