Application of aeration-assisted homogeneous liquid-liquid microextraction procedure using Box-Behnken design for determination of curcumin by HPLC.

ABSTRACT

A simple, efficient, and rapid sample preparation method based on aeration-assisted homogeneous liquid-liquid microextraction was developed for determination of curcumin in food samples by high-performance liquid chromatography. The centrifuge step has been eliminated in this procedure. The effects of some variables, such as pH, volume of extraction solvent, extraction time, and salt effect, were studied through a Box-Behnken design method. Under the optimum conditions, calibration curves of curcumin were linear in the range of 0.08-4000 µg/mL with R2  = 0.997. Limit of detection and relative standard deviation were 0.019 µg/mL and 3.01%, respectively. The preconcentration factor achieved was 166. The proposed method was successfully applied to determination of curcumin in various food samples.