Your browser doesn't support javascript.
loading
A CRISPR-based assay for the detection of opportunistic infections post-transplantation and for the monitoring of transplant rejection.
Kaminski, Michael M; Alcantar, Miguel A; Lape, Isadora T; Greensmith, Robert; Huske, Allison C; Valeri, Jacqueline A; Marty, Francisco M; Klämbt, Verena; Azzi, Jamil; Akalin, Enver; Riella, Leonardo V; Collins, James J.
Affiliation
  • Kaminski MM; Institute for Medical Engineering and Science and Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Alcantar MA; Berlin Institute for Medical Systems Biology, Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany.
  • Lape IT; Department of Nephrology and Medical Intensive Care, Charité-Universitätsmedizin Berlin, Berlin, Germany.
  • Greensmith R; Institute for Medical Engineering and Science and Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Huske AC; Schuster Transplantation Research Center, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
  • Valeri JA; Berlin Institute for Medical Systems Biology, Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany.
  • Marty FM; Department of Nephrology and Medical Intensive Care, Charité-Universitätsmedizin Berlin, Berlin, Germany.
  • Klämbt V; Institute for Medical Engineering and Science and Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Azzi J; Institute for Medical Engineering and Science and Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Akalin E; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA.
  • Riella LV; Division of Infectious Diseases, Brigham and Women's Hospital, Boston, MA, USA.
  • Collins JJ; Department of Medicine, Boston Children's Hospital, Harvard Medical School, Boston, MA, USA.
Nat Biomed Eng ; 4(6): 601-609, 2020 06.
Article in En | MEDLINE | ID: mdl-32284553
ABSTRACT
In organ transplantation, infection and rejection are major causes of graft loss. They are linked by the net state of immunosuppression. To diagnose and treat these conditions earlier, and to improve long-term patient outcomes, refined strategies for the monitoring of patients after graft transplantation are needed. Here, we show that a fast and inexpensive assay based on CRISPR-Cas13 accurately detects BK polyomavirus DNA and cytomegalovirus DNA from patient-derived blood and urine samples, as well as CXCL9 messenger RNA (a marker of graft rejection) at elevated levels in urine samples from patients experiencing acute kidney transplant rejection. The assay, which we adapted for lateral-flow readout, enables-via simple visualization-the post-transplantation monitoring of common opportunistic viral infections and of graft rejection, and should facilitate point-of-care post-transplantation monitoring.
Subject(s)
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Opportunistic Infections / Pathology, Molecular / CRISPR-Cas Systems / Graft Rejection Type of study: Diagnostic_studies Limits: Humans / Male / Middle aged Language: En Journal: Nat Biomed Eng Year: 2020 Document type: Article Affiliation country: United States
Fulltext
Add to My VHL
Print
XML
PubMed Links
Search on Google

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Opportunistic Infections / Pathology, Molecular / CRISPR-Cas Systems / Graft Rejection Type of study: Diagnostic_studies Limits: Humans / Male / Middle aged Language: En Journal: Nat Biomed Eng Year: 2020 Document type: Article Affiliation country: United States