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Smartphone-based multiplex 30-minute nucleic acid test of live virus from nasal swab extract.
Sun, Fu; Ganguli, Anurup; Nguyen, Judy; Brisbin, Ryan; Shanmugam, Krithika; Hirschberg, David L; Wheeler, Matthew B; Bashir, Rashid; Nash, David M; Cunningham, Brian T.
Affiliation
  • Sun F; Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Illinois, USA. bcunning@illinois.edu.
  • Ganguli A; Department of Bioengineering, University of Illinois at Urbana-Champaign, Illinois, USA.
  • Nguyen J; RAIN Incubator, Tacoma, Washington, USA.
  • Brisbin R; Department of Interdisciplinary Arts and Sciences & The Center for Urban Waters, University of Washington Tacoma, Washington, USA.
  • Shanmugam K; Department of Interdisciplinary Arts and Sciences & The Center for Urban Waters, University of Washington Tacoma, Washington, USA.
  • Hirschberg DL; RAIN Incubator, Tacoma, Washington, USA and Department of Interdisciplinary Arts and Sciences & The Center for Urban Waters, University of Washington Tacoma, Washington, USA.
  • Wheeler MB; Department of Animal Sciences, University of Illinois at Urbana-Champaign, Illinois, USA.
  • Bashir R; Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Illinois, USA. bcunning@illinois.edu and Department of Bioengineering, University of Illinois at Urbana-Champaign, Illinois, USA.
  • Nash DM; Private equine veterinarian, Kentucky, USA.
  • Cunningham BT; Department of Electrical and Computer Engineering, University of Illinois at Urbana-Champaign, Illinois, USA. bcunning@illinois.edu and Department of Bioengineering, University of Illinois at Urbana-Champaign, Illinois, USA.
Lab Chip ; 20(9): 1621-1627, 2020 05 05.
Article in En | MEDLINE | ID: mdl-32334422
ABSTRACT
Rapid, sensitive and specific detection and reporting of infectious pathogens is important for patient management and epidemic surveillance. We demonstrated a point-of-care system integrated with a smartphone for detecting live virus from nasal swab media, using a panel of equine respiratory infectious diseases as a model system for corresponding human diseases such as COVID-19. Specific nucleic acid sequences of five pathogens were amplified by loop-mediated isothermal amplification on a microfluidic chip and detected at the end of reactions by the smartphone. Pathogen-spiked horse nasal swab samples were correctly diagnosed using our system, with a limit of detection comparable to that of the traditional lab-based test, polymerase chain reaction, with results achieved in ∼30 minutes.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Respiration Disorders / Nucleic Acid Amplification Techniques / Molecular Diagnostic Techniques / Lab-On-A-Chip Devices / Smartphone / Horse Diseases Type of study: Diagnostic_studies / Prognostic_studies Limits: Animals Language: En Journal: Lab Chip Journal subject: BIOTECNOLOGIA / QUIMICA Year: 2020 Document type: Article Affiliation country: United States
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Respiration Disorders / Nucleic Acid Amplification Techniques / Molecular Diagnostic Techniques / Lab-On-A-Chip Devices / Smartphone / Horse Diseases Type of study: Diagnostic_studies / Prognostic_studies Limits: Animals Language: En Journal: Lab Chip Journal subject: BIOTECNOLOGIA / QUIMICA Year: 2020 Document type: Article Affiliation country: United States