Modulation of Peptidoglycan Synthesis by Recycled Cell Wall Tetrapeptides.
Cell Rep
; 31(4): 107578, 2020 04 28.
Article
in En
| MEDLINE
| ID: mdl-32348759
ABSTRACT
The bacterial cell wall is made of peptidoglycan (PG), a polymer that is essential for the maintenance of cell shape and survival. During growth, bacteria remodel their PG, releasing fragments that are predominantly re-internalized and recycled. Here, we show that Vibrio cholerae recycles PG fragments modified with non-canonical d-amino acids (NCDAA), which lead to the accumulation of cytosolic PG tetrapeptides. We demonstrate that the accumulation of recycled tetrapeptides has two regulatory consequences for the cell wall reduction of d,d-cross-linkage and reduction of PG synthesis. We further demonstrate that l,d-carboxypeptidases from five different species show a preferential activity for substrates containing canonical (d-alanine) versus non-canonical (d-methionine) d-amino acids, suggesting that the accumulation of intracellular tetrapeptides in NCDAA-rich environments is widespread. Collectively, this work reveals a regulatory role of NCDAA linking PG recycling and synthesis to promote optimal cell wall assembly and composition in the stationary phase.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Peptidoglycan
/
Cell Wall
Language:
En
Journal:
Cell Rep
Year:
2020
Document type:
Article
Affiliation country:
Sweden