N-Terminal speciation for native chemical ligation.
Chem Commun (Camb)
; 56(45): 6114-6117, 2020 Jun 07.
Article
in En
| MEDLINE
| ID: mdl-32363374
ABSTRACT
Native chemical ligation (NCL) enables the chemical synthesis of peptides via reactions between N-terminal thiolates and C-terminal thioesters under mild, aqueous conditions at pH 7-8. Here we demonstrate quantitatively how thiol speciation at N-terminal cysteines and analogues varies significantly depending upon structure at typical pH values used in NCL.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Language:
En
Journal:
Chem Commun (Camb)
Journal subject:
QUIMICA
Year:
2020
Document type:
Article
Affiliation country:
United kingdom