Chemical study, antioxidant activity, and genotoxicity and cytotoxicity evaluation of Ruellia angustiflora.
Nat Prod Res
; 35(23): 5317-5322, 2021 Dec.
Article
in En
| MEDLINE
| ID: mdl-32363928
ABSTRACT
Ruellia angustiflora (Acanthaceae) is known as flower-of-fire, and its leaves are traditionally employed to promote wound healing. This study was aimed at extracting and characterizing the chemical constituents of the extracts of R. angustiflora obtained by ultrasound-assisted and supercritical fluid extractions, and subsequently investigate their antioxidant potential and cyto-genotoxicity. The extract obtained by ultrasound (UAE-EtOH) was characterized by ultraperformance liquid chromatography-mass spectrometer (UPLC-MS), and the extract obtained via supercritical fluid (SFE-CO2) by gas chromatograph-mass spectrometer (GC-MS). The antioxidant potential was verified by the antiradical activity against the 1,1-diphenyl-2-picrylhydrazyl radical (DPPH), and the cyto-genotoxicity evaluation to test cell viability and DNA damage was performed in peripheral blood mononuclear cells (PBMC) cultures. The SFE-CO2 extract presented some fatty acids, triterpenes, tetraterpenes, tocopherols and phytosterols. The UAE-EtOH extract contained phenolic acids and flavonoids, and showed the highest antioxidant capacity. Neither extract was genotoxic or cytotoxic at the tested concentrations.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Acanthaceae
/
Antioxidants
Language:
En
Journal:
Nat Prod Res
Year:
2021
Document type:
Article
Affiliation country:
Brazil