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Characterization of a novel automated microfiltration device for the efficient isolation and analysis of circulating tumor cells from clinical blood samples.
Yee-de León, Juan F; Soto-García, Brenda; Aráiz-Hernández, Diana; Delgado-Balderas, Jesús Rolando; Esparza, Miguel; Aguilar-Avelar, Carlos; Wong-Campos, J D; Chacón, Franco; López-Hernández, José Y; González-Treviño, A Mauricio; Yee-de León, José R; Zamora-Mendoza, Jorge L; Alvarez, Mario M; Trujillo-de Santiago, Grissel; Gómez-Guerra, Lauro S; Sánchez-Domínguez, Celia N; Velarde-Calvillo, Liza P; Abarca-Blanco, Alejandro.
Affiliation
  • Yee-de León JF; Delee Corp., Mountain View, CA, 94041, USA.
  • Soto-García B; Delee Corp., Mountain View, CA, 94041, USA.
  • Aráiz-Hernández D; Delee Corp., Mountain View, CA, 94041, USA.
  • Delgado-Balderas JR; Delee Corp., Mountain View, CA, 94041, USA.
  • Esparza M; Departamento de Bioquímica y Medicina Molecular, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey, 64460, Mexico.
  • Aguilar-Avelar C; Delee Corp., Mountain View, CA, 94041, USA.
  • Wong-Campos JD; Delee Corp., Mountain View, CA, 94041, USA.
  • Chacón F; Delee Corp., Mountain View, CA, 94041, USA.
  • López-Hernández JY; Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, 02138, USA.
  • González-Treviño AM; Delee Corp., Mountain View, CA, 94041, USA.
  • Yee-de León JR; Delee Corp., Mountain View, CA, 94041, USA.
  • Zamora-Mendoza JL; Delee Corp., Mountain View, CA, 94041, USA.
  • Alvarez MM; Delee Corp., Mountain View, CA, 94041, USA.
  • Trujillo-de Santiago G; Delee Corp., Mountain View, CA, 94041, USA.
  • Gómez-Guerra LS; Centro de Biotecnología-FEMSA, Escuela de Ingeniería y Ciencias, Tecnologico de Monterrey, Monterrey, 64849, Mexico.
  • Sánchez-Domínguez CN; Departamento de Bioingeniería, Escuela de Ingeniería y Ciencias, Tecnologico de Monterrey, Monterrey, 64849, Mexico.
  • Velarde-Calvillo LP; Centro de Biotecnología-FEMSA, Escuela de Ingeniería y Ciencias, Tecnologico de Monterrey, Monterrey, 64849, Mexico.
  • Abarca-Blanco A; Departamento de Mecatrónica e Ingeniería Eléctrica, Escuela de Ingeniería y Ciencias, Tecnologico de Monterrey, Monterrey, 64849, Mexico.
Sci Rep ; 10(1): 7543, 2020 05 05.
Article in En | MEDLINE | ID: mdl-32372001
The detection and analysis of circulating tumor cells (CTCs) may enable a broad range of cancer-related applications, including the identification of acquired drug resistance during treatments. However, the non-scalable fabrication, prolonged sample processing times, and the lack of automation, associated with most of the technologies developed to isolate these rare cells, have impeded their transition into the clinical practice. This work describes a novel membrane-based microfiltration device comprised of a fully automated sample processing unit and a machine-vision-enabled imaging system that allows the efficient isolation and rapid analysis of CTCs from blood. The device performance was characterized using four prostate cancer cell lines, including PC-3, VCaP, DU-145, and LNCaP, obtaining high assay reproducibility and capture efficiencies greater than 93% after processing 7.5 mL blood samples spiked with 100 cancer cells. Cancer cells remained viable after filtration due to the minimal shear stress exerted over cells during the procedure, while the identification of cancer cells by immunostaining was not affected by the number of non-specific events captured on the membrane. We were also able to identify the androgen receptor (AR) point mutation T878A from 7.5 mL blood samples spiked with 50 LNCaP cells using RT-PCR and Sanger sequencing. Finally, CTCs were detected in 8 out of 8 samples from patients diagnosed with metastatic prostate cancer (mean ± SEM = 21 ± 2.957 CTCs/mL, median = 21 CTCs/mL), demonstrating the potential clinical utility of this device.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Prostatic Neoplasms / Cell Separation / Filtration / Neoplastic Cells, Circulating Type of study: Prognostic_studies Limits: Adult / Aged / Aged80 / Humans / Male / Middle aged Language: En Journal: Sci Rep Year: 2020 Document type: Article Affiliation country: United States Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Prostatic Neoplasms / Cell Separation / Filtration / Neoplastic Cells, Circulating Type of study: Prognostic_studies Limits: Adult / Aged / Aged80 / Humans / Male / Middle aged Language: En Journal: Sci Rep Year: 2020 Document type: Article Affiliation country: United States Country of publication: United kingdom