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Co-immunization of DNA and Protein in the Same Anatomical Sites Induces Superior Protective Immune Responses against SHIV Challenge.
Felber, Barbara K; Lu, Zhongyan; Hu, Xintao; Valentin, Antonio; Rosati, Margherita; Remmel, Christopher A L; Weiner, Joshua A; Carpenter, Margaret C; Faircloth, Katelyn; Stanfield-Oakley, Sherry; Williams, Wilton B; Shen, Xiaoying; Tomaras, Georgia D; LaBranche, Celia C; Montefiori, David; Trinh, Hung V; Rao, Mangala; Alam, Munir S; Vandergrift, Nathan A; Saunders, Kevin O; Wang, Yunfei; Rountree, Wes; Das, Jishnu; Alter, Galit; Reed, Steven G; Aye, Pyone P; Schiro, Faith; Pahar, Bapi; Dufour, Jason P; Veazey, Ronald S; Marx, Preston A; Venzon, David J; Shaw, George M; Ferrari, Guido; Ackerman, Margaret E; Haynes, Barton F; Pavlakis, George N.
Affiliation
  • Felber BK; Human Retrovirus Pathogenesis Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, MD 21702, USA. Electronic address: barbara.felber@nih.gov.
  • Lu Z; Human Retrovirus Pathogenesis Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, MD 21702, USA.
  • Hu X; Human Retrovirus Pathogenesis Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, MD 21702, USA.
  • Valentin A; Human Retrovirus Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, MD 21702, USA.
  • Rosati M; Human Retrovirus Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, MD 21702, USA.
  • Remmel CAL; Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA.
  • Weiner JA; Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA.
  • Carpenter MC; Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA.
  • Faircloth K; Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA.
  • Stanfield-Oakley S; Duke Human Vaccine Institute, Duke University, Durham, NC 27710, USA; Department of Surgery, Duke University, Durham, NC 27710, USA; Department of Molecular Genetics and Microbiology, Duke University, Durham, NC 27710, USA.
  • Williams WB; Duke Human Vaccine Institute, Duke University, Durham, NC 27710, USA.
  • Shen X; Duke Human Vaccine Institute, Duke University, Durham, NC 27710, USA.
  • Tomaras GD; Duke Human Vaccine Institute, Duke University, Durham, NC 27710, USA; Department of Surgery, Duke University, Durham, NC 27710, USA; Department of Molecular Genetics and Microbiology, Duke University, Durham, NC 27710, USA; Department of Immunology, Duke University, Durham, NC 27710, USA.
  • LaBranche CC; Duke Human Vaccine Institute, Duke University, Durham, NC 27710, USA; Department of Surgery, Duke University, Durham, NC 27710, USA.
  • Montefiori D; Duke Human Vaccine Institute, Duke University, Durham, NC 27710, USA; Department of Surgery, Duke University, Durham, NC 27710, USA.
  • Trinh HV; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA; Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, MD 20817, USA.
  • Rao M; U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, MD 20910, USA.
  • Alam MS; Duke Human Vaccine Institute, Duke University, Durham, NC 27710, USA.
  • Vandergrift NA; Duke Human Vaccine Institute, Duke University, Durham, NC 27710, USA.
  • Saunders KO; Duke Human Vaccine Institute, Duke University, Durham, NC 27710, USA; Department of Surgery, Duke University, Durham, NC 27710, USA.
  • Wang Y; Duke Human Vaccine Institute, Duke University, Durham, NC 27710, USA.
  • Rountree W; Duke Human Vaccine Institute, Duke University, Durham, NC 27710, USA.
  • Das J; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA 02139, USA.
  • Alter G; Ragon Institute of MGH, MIT and Harvard, Cambridge, MA 02139, USA.
  • Reed SG; Infectious Disease Research Institute, Seattle, WA 98102, USA.
  • Aye PP; Division of Comparative Pathology, Tulane National Primate Research Center, Covington, LA 70433, USA.
  • Schiro F; Division of Comparative Pathology, Tulane National Primate Research Center, Covington, LA 70433, USA.
  • Pahar B; Division of Comparative Pathology, Tulane National Primate Research Center, Covington, LA 70433, USA.
  • Dufour JP; Division of Comparative Pathology, Tulane National Primate Research Center, Covington, LA 70433, USA.
  • Veazey RS; Division of Comparative Pathology, Tulane National Primate Research Center, Covington, LA 70433, USA.
  • Marx PA; Tulane National Primate Research Center, and Department of Tropical Medicine, School of Public Health and Tropical Medicine, Tulane University, New Orleans, LA 70112, USA.
  • Venzon DJ; Biostatistics and Data Management Section, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.
  • Shaw GM; Departments of Medicine and Microbiology, University of Pennsylvania, Philadelphia, PA 19104, USA.
  • Ferrari G; Duke Human Vaccine Institute, Duke University, Durham, NC 27710, USA; Department of Surgery, Duke University, Durham, NC 27710, USA; Department of Molecular Genetics and Microbiology, Duke University, Durham, NC 27710, USA.
  • Ackerman ME; Thayer School of Engineering, Dartmouth College, Hanover, NH 03755, USA.
  • Haynes BF; Duke Human Vaccine Institute, Duke University, Durham, NC 27710, USA; Department of Medicine, Duke University Medical Center, Durham, NC 27710, USA. Electronic address: barton.haynes@duke.edu.
  • Pavlakis GN; Human Retrovirus Section, Vaccine Branch, Center for Cancer Research, National Cancer Institute at Frederick, Frederick, MD 21702, USA. Electronic address: george.pavlakis@nih.gov.
Cell Rep ; 31(6): 107624, 2020 05 12.
Article in En | MEDLINE | ID: mdl-32402293
ABSTRACT
We compare immunogenicity and protective efficacy of an HIV vaccine comprised of env and gag DNA and Env (Envelope) proteins by co-administration of the vaccine components in the same muscles or by separate administration of DNA + protein in contralateral sites in female rhesus macaques. The 6-valent vaccine includes gp145 Env DNAs, representing six sequentially isolated Envs from the HIV-infected individual CH505, and matching GLA-SE-adjuvanted gp120 Env proteins. Interestingly, only macaques in the co-administration vaccine group are protected against SHIV CH505 acquisition after repeated low-dose intravaginal challenge and show 67% risk reduction per exposure. Macaques in the co-administration group develop higher Env-specific humoral and cellular immune responses. Non-neutralizing Env antibodies, ADCC, and antibodies binding to FcγRIIIa are associated with decreased transmission risk. These data suggest that simultaneous recognition, processing, and presentation of DNA + Env protein in the same draining lymph nodes play a critical role in the development of protective immunity.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA / Proteins / Immunization / Simian Immunodeficiency Virus / Macaca Limits: Animals / Humans Language: En Journal: Cell Rep Year: 2020 Document type: Article
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA / Proteins / Immunization / Simian Immunodeficiency Virus / Macaca Limits: Animals / Humans Language: En Journal: Cell Rep Year: 2020 Document type: Article