Recombinant PAL/PilE/FlaA DNA vaccine provides protective immunity against Legionella pneumophila in BALB/c mice.
BMC Biotechnol
; 20(1): 28, 2020 05 18.
Article
in En
| MEDLINE
| ID: mdl-32423439
ABSTRACT
BACKGROUND:
Legionella pneumophila (L.pneumophila), a Gram-negative small microorganism, causes hospital-acquired pneumonia especially in immunocompromised patients. Vaccination may be an effective method for preventing L.pneumophila infection. Therefore, it is necessary to develop a better vaccine against this disease. In this study, we developed a recombinant peptidoglycan-associated lipoprotein (PAL)/type IV pilin (PilE)/lagellin (FlaA) DNA vaccine and evaluated its immunogenicity and efficacy to protect against L.pneumophila infection.RESULTS:
According to the results, the expression of PAL, PilE, FlaA proteins and PAL/PilE/FlaA fusion protein in 293 cells was confirmed. Immunization with PAL/PilE/FlaA DNA vaccine resulted in highest IgG titer and strongest cytotoxic T-lymphocyte (CTL) response. Furthermore, the histopathological changes in lung tissues of mice challenged with a lethal dose of L.pneumophila were alleviated by PAL/PilE/FlaA DNA vaccine immunization. The production of T-helper-1 (Th1) cytokines (IFNγ, TGF-α, and IL-12), and Th2 cytokines (IL-4 and IL-10) were promoted in PAL/PilE/FlaA DNA vaccine group. Finally, immunization with PAL/PilE/FlaA vaccine raised the survival rate of mice to 100% after challenging with a lethal dose of L.pneumophila for 10 consecutive days.CONCLUSIONS:
Our study suggests that the newly developed PAL/PilE/FlaA DNA vaccine stimulates strong humoral and cellular immune responses and may be a potential intervention on L.pneumophila infection.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Legionnaires' Disease
/
Bacterial Vaccines
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Peptidoglycan
/
Legionella pneumophila
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Vaccines, DNA
/
Fimbriae Proteins
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Flagellin
/
Lipoproteins
Limits:
Animals
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Female
/
Humans
Language:
En
Journal:
BMC Biotechnol
Journal subject:
BIOTECNOLOGIA
Year:
2020
Document type:
Article