Delayed Laboratory Response to COVID-19 Caused by Molecular Diagnostic Contamination.

Mögling, Ramona; Meijer, Adam; Berginc, Natasa; Bruisten, Sylvia; Charrel, Remi; Coutard, Bruno; Eckerle, Isabella; Enouf, Vincent; Hungnes, Olav; Korukluoglu, Gülay; Kossyvakis, Thanos; Mentis, Andreas; Molenkamp, Richard; Muradrasoli, Shaman; Papa, Anna; Pigny, Fiona; Thirion, Laurence; van der Werf, Sylvie; Reusken, Chantal

Mögling, Ramona; Meijer, Adam; Berginc, Natasa; Bruisten, Sylvia; Charrel, Remi; Coutard, Bruno; Eckerle, Isabella; Enouf, Vincent; Hungnes, Olav; Korukluoglu, Gülay; Kossyvakis, Thanos; Mentis, Andreas; Molenkamp, Richard; Muradrasoli, Shaman; Papa, Anna; Pigny, Fiona; Thirion, Laurence; van der Werf, Sylvie; Reusken, Chantal.

Emerg Infect Dis ; 26(8): 1944-1946, 2020 08.

Article in En | MEDLINE | ID: mdl-32433015

ABSTRACT

ABSTRACT

The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) created an exceptional situation in which numerous laboratories in Europe simultaneously implemented SARS-CoV-2 diagnostics. These laboratories reported in February 2020 that commercial primer and probe batches for SARS-CoV-2 detection were contaminated with synthetic control material, causing delays of regional testing roll-out in various countries.

Subject(s)

Artifacts; Betacoronavirus/genetics; Clinical Laboratory Techniques/standards; Coronavirus Infections/diagnosis; Coronavirus Infections/epidemiology; Pneumonia, Viral/diagnosis; Pneumonia, Viral/epidemiology; Reagent Kits, Diagnostic/standards; Reverse Transcriptase Polymerase Chain Reaction/standards; Betacoronavirus/pathogenicity; COVID-19; COVID-19 Testing; Clinical Laboratory Techniques/methods; Coronavirus Envelope Proteins; Coronavirus Infections/virology; DNA Primers/analysis; DNA Primers/chemical synthesis; DNA Probes/analysis; DNA Probes/chemical synthesis; Delayed Diagnosis; Diagnostic Tests, Routine; Europe/epidemiology; Humans; Laboratories/organization & administration; Laboratories/standards; Pandemics; Pathology, Molecular; Pneumonia, Viral/virology; RNA-Dependent RNA Polymerase/genetics; Reagent Kits, Diagnostic/supply & distribution; Reverse Transcriptase Polymerase Chain Reaction/statistics & numerical data; SARS-CoV-2; Viral Envelope Proteins/genetics

Key words

COVID-19; Europe; PCR; SARS-CoV-2; commercial nucleotide production; contamination; coronavirus disease; coronaviruses; delayed laboratory response; molecular diagnostics; primers; probes; respiratory infections; severe acute respiratory syndrome coronavirus 2; viruses; zoonoses

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pneumonia, Viral / Reagent Kits, Diagnostic / Artifacts / Coronavirus Infections / Clinical Laboratory Techniques / Reverse Transcriptase Polymerase Chain Reaction / Betacoronavirus Limits: Humans Country/Region as subject: Europa Language: En Journal: Emerg Infect Dis Journal subject: DOENCAS TRANSMISSIVEIS Year: 2020 Document type: Article

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