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Four Phosphates at One Blow: Access to Pentaphosphorylated Magic Spot Nucleotides and Their Analysis by Capillary Electrophoresis.
Haas, Thomas M; Qiu, Danye; Häner, Markus; Angebauer, Larissa; Ripp, Alexander; Singh, Jyoti; Koch, Hans-Georg; Jessen-Trefzer, Claudia; Jessen, Henning J.
Affiliation
  • Haas TM; Institute of Organic Chemistry, University of Freiburg, 79104 Freiburg, Germany.
  • Qiu D; Institute of Organic Chemistry, University of Freiburg, 79104 Freiburg, Germany.
  • Häner M; Institute of Organic Chemistry, University of Freiburg, 79104 Freiburg, Germany.
  • Angebauer L; Institute of Biochemistry and Molecular Biology, Faculty of Medicine, University of Freiburg, 79104 Freiburg, Germany.
  • Ripp A; Faculty of Biology, University of Freiburg, 79104 Freiburg, Germany.
  • Singh J; Institute of Organic Chemistry, University of Freiburg, 79104 Freiburg, Germany.
  • Koch HG; Institute of Organic Chemistry, University of Freiburg, 79104 Freiburg, Germany.
  • Jessen-Trefzer C; Institute of Biochemistry and Molecular Biology, Faculty of Medicine, University of Freiburg, 79104 Freiburg, Germany.
  • Jessen HJ; Institute of Pharmaceutical Biology and Biotechnology, University of Freiburg, 79104 Freiburg, Germany.
J Org Chem ; 85(22): 14496-14506, 2020 11 20.
Article in En | MEDLINE | ID: mdl-32502348
The complex phosphorylation pattern of natural and modified pentaphosphorylated magic spot nucleotides is generated in a highly efficient way. A cyclic pyrophosphoryl phosphoramidite (cPyPA) reagent is used to introduce four phosphates on nucleosides regioselectively in a one-flask key transformation. The obtained magic spot nucleotides are used to develop a capillary electrophoresis UV detection method, enabling nucleotide assignment in complex bacterial extracts.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: J Org Chem Year: 2020 Document type: Article Affiliation country: Germany Country of publication: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: J Org Chem Year: 2020 Document type: Article Affiliation country: Germany Country of publication: United States