Uncovering the chemistry of C-C bond formation in C-nucleoside biosynthesis: crystal structure of a C-glycoside synthase/PRPP complex.
Chem Commun (Camb)
; 56(55): 7617-7620, 2020 Jul 14.
Article
in En
| MEDLINE
| ID: mdl-32515440
ABSTRACT
The enzyme ForT catalyzes C-C bond formation between 5'-phosphoribosyl-1'-pyrophosphate (PRPP) and 4-amino-1H-pyrazole-3,5-dicarboxylate to make a key intermediate in the biosynthesis of formycin A 5'-phosphate by Streptomyces kaniharaensis. We report the 2.5 Å resolution structure of the ForT/PRPP complex and locate active site residues critical for PRPP recognition and catalysis.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phosphoribosyl Pyrophosphate
/
Carbon-Carbon Ligases
Language:
En
Journal:
Chem Commun (Camb)
Journal subject:
QUIMICA
Year:
2020
Document type:
Article
Affiliation country:
United kingdom