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DNA double strand break repair as cellular response to genotoxic asarone isomers considering phase I metabolism.
Hermes, Lena; Haupenthal, Sabrina; Uebel, Thomas; Esselen, Melanie.
Affiliation
  • Hermes L; University of Muenster, Institute of Food Chemistry, Corrensstraße 45, 48149, Muenster, Germany.
  • Haupenthal S; University of Muenster, Institute of Food Chemistry, Corrensstraße 45, 48149, Muenster, Germany.
  • Uebel T; University of Muenster, Institute of Food Chemistry, Corrensstraße 45, 48149, Muenster, Germany.
  • Esselen M; University of Muenster, Institute of Food Chemistry, Corrensstraße 45, 48149, Muenster, Germany. Electronic address: esselen@uni-muenster.de.
Food Chem Toxicol ; 142: 111484, 2020 Aug.
Article in En | MEDLINE | ID: mdl-32526244
The phenylpropenes α-asarone and ß-asarone are widely spread in the marsh plant Acorus calamus. Both isomers are classified as carcinogenic in rodents. However, the respective genotoxic mechanisms are not elucidated so far. The present study gives deeper insights into the genotoxic effects of asarone isomers as well as their known oxidative phase I metabolites, (E)-3'-oxoasarone and asarone epoxide. We show that asarone metabolites highly increase DNA strand breaks after 1 h of incubation, markedly metabolic activation contributes to their carcinogenic mode of action. All test compounds act as aneugens and potently enhance the amounts of micronuclei in binuclear cells. However, a prolonged incubation time of 24 h results in a decrease of DNA damage. This work suggests that asarone metabolites also induce DNA double strand breaks , why we put a strong focus on homologous recombination and non-homologous end joining. The obtained results herein indicate that asarone epoxide-induced DNA strand breaks are repaired via a homologous repair pathway.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA Breaks, Double-Stranded / Anisoles / Mutagens Limits: Humans Language: En Journal: Food Chem Toxicol Year: 2020 Document type: Article Affiliation country: Germany Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA Breaks, Double-Stranded / Anisoles / Mutagens Limits: Humans Language: En Journal: Food Chem Toxicol Year: 2020 Document type: Article Affiliation country: Germany Country of publication: United kingdom