Association of nitric oxide synthase 3 gene rs1799983 G/T polymorphism with idiopathic asthenozoospermia in Iranian Azeri males: a case-control study.
Horm Mol Biol Clin Investig
; 41(4)2020 Jun 29.
Article
in En
| MEDLINE
| ID: mdl-32598309
ABSTRACT
OBJECTIVES:
Recently, oxidative stress (OS) has been described extensively as an important cause of men infertility. The nitric oxide synthase 3 (NOS3) gene expression involved in normal spermatogenesis regulation in testis. Several single nucleotide polymorphisms (SNPs) on NOS3 gene are reported in association with sperm function and spermatogenesis impairment in infertile men. In present study, we investigated association of NOS3 gene rs1799983 G/T polymorphism in Iranian Azeri male with idiopathic asthenozoospermia (AZS).METHODS:
In this case-control study, we collected 50 males with idiopathic AZS as a case group and 50 age and ethnically matched male as healthy controls from East Azerbaijan area, Iran. The case and control groups genotyping was performed using tetra-primer amplification refractory mutation system-polymerase chain reaction (Tetra-ARMS PCR) method.RESULTS:
Genotype frequency in AZS patients was 40% GG, 60% GT, and 0% TT, whereas in healthy controls were 60% GG, 30% GT, and 10% TT. Statistical analysis showed that the GT heterozygous genotype frequency of NOS3 gene rs1799983 G/T polymorphism in AZS patients was significantly more than healthy controls (p>0.05).CONCLUSIONS:
We demonstrated that NOS3 gene rs1799983 G/T polymorphism was associated with AZS in Iranian Azeri men. However, more studies on different geographic areas, races and ethnicities are required to determine exact role of NOS3 gene rs1799983 G/T polymorphism in idiopathic AZS.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Genetic Predisposition to Disease
/
Polymorphism, Single Nucleotide
/
Alleles
/
Nitric Oxide Synthase Type III
/
Asthenozoospermia
Type of study:
Diagnostic_studies
/
Observational_studies
/
Risk_factors_studies
Limits:
Humans
/
Male
Country/Region as subject:
Asia
Language:
En
Journal:
Horm Mol Biol Clin Investig
Year:
2020
Document type:
Article
Affiliation country:
Iran