[Toxicity of Soil Leachate from Coal Gangue and Its Surrounding Village of Barley(Hordeum vulgare)].

Coal gangue is a harmful solid waste product of coal mining. When it accumulates for a long time, it becomes harmful to the surroundings. To investigate the adverse effect of coal gangue on the surrounding environment, this study investigated the effects of coal gangue and its downstream village on the growth toxicity and genotoxicity of barley at different dilution concentrations (1：27, 1：9, 1：3, and 1：1) via hydroponic experiments. As a result, low concentration coal gangue showed a slight promotion effect on the growth of roots and shoots of barley, while coal gangue and village soil, which have a high concentration, could seriously inhibit their germination and growth. At the same time, with the increase of the concentration of coal gangue, malondialdehyde (MDA) in barley leaves increased, and chlorophyll (Chl) increased first and then decreased, while the village soil showed a lower toxic effect. In addition, our results showed that higher concentrations of coal gangue and village soil could decrease the mitotic index and increase the micronucleus rate in root tip cells, indicating that the toxicity mechanism of coal gangue to barley may be involved in genotoxicity. These results provide experimental evidence for the ecological risk assessment of the coal gangue and its surrounding environment.