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Cryo-EM structure of arabinosyltransferase EmbB from Mycobacterium smegmatis.
Tan, Yong Zi; Rodrigues, José; Keener, James E; Zheng, Ruixiang Blake; Brunton, Richard; Kloss, Brian; Giacometti, Sabrina I; Rosário, Ana L; Zhang, Lei; Niederweis, Michael; Clarke, Oliver B; Lowary, Todd L; Marty, Michael T; Archer, Margarida; Potter, Clinton S; Carragher, Bridget; Mancia, Filippo.
Affiliation
  • Tan YZ; Department of Physiology and Cellular Biophysics, Columbia University, New York, NY, 10032, USA.
  • Rodrigues J; National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology Center, New York, NY, 10027, USA.
  • Keener JE; Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB NOVA), 2780-157, Oeiras, Portugal.
  • Zheng RB; Department of Chemistry and Biochemistry, University of Arizona, Tucson, AZ, 85721, USA.
  • Brunton R; Department of Chemistry, University of Alberta, Edmonton, Alberta, T6G 2G2, Canada.
  • Kloss B; Department of Chemistry, University of Alberta, Edmonton, Alberta, T6G 2G2, Canada.
  • Giacometti SI; Center on Membrane Protein Production and Analysis, New York Structural Biology Center, New York, NY, 10027, USA.
  • Rosário AL; Department of Physiology and Cellular Biophysics, Columbia University, New York, NY, 10032, USA.
  • Zhang L; Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa (ITQB NOVA), 2780-157, Oeiras, Portugal.
  • Niederweis M; Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL, 35294, USA.
  • Clarke OB; Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL, 35294, USA.
  • Lowary TL; Department of Physiology and Cellular Biophysics, Columbia University, New York, NY, 10032, USA.
  • Marty MT; Department of Anesthesiology, Columbia University, New York, NY, 10032, USA.
  • Archer M; Department of Chemistry, University of Alberta, Edmonton, Alberta, T6G 2G2, Canada.
  • Potter CS; Institute of Biological Chemistry, Academia Sinica, Academia Road, Section 2, #128, Nangang, Taipei, 11529, Taiwan.
  • Carragher B; Department of Chemistry and Biochemistry, University of Arizona, Tucson, AZ, 85721, USA.
  • Mancia F; Bio5 Institute, University of Arizona, Tucson, AZ, 85721, USA.
Nat Commun ; 11(1): 3396, 2020 07 07.
Article in En | MEDLINE | ID: mdl-32636380
ABSTRACT
Arabinosyltransferase B (EmbB) belongs to a family of membrane-bound glycosyltransferases that build the lipidated polysaccharides of the mycobacterial cell envelope, and are targets of anti-tuberculosis drug ethambutol. We present the 3.3 Å resolution single-particle cryo-electron microscopy structure of Mycobacterium smegmatis EmbB, providing insights on substrate binding and reaction mechanism. Mutations that confer ethambutol resistance map mostly around the putative active site, suggesting this to be the location of drug binding.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pentosyltransferases / Mycobacterium smegmatis Language: En Journal: Nat Commun Journal subject: BIOLOGIA / CIENCIA Year: 2020 Document type: Article Affiliation country: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Pentosyltransferases / Mycobacterium smegmatis Language: En Journal: Nat Commun Journal subject: BIOLOGIA / CIENCIA Year: 2020 Document type: Article Affiliation country: United States