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Random epigenetic modulation of CHO cells by repeated knockdown of DNA methyltransferases increases population diversity and enables sorting of cells with higher production capacities.
Weinguny, Marcus; Eisenhut, Peter; Klanert, Gerald; Virgolini, Nikolaus; Marx, Nicolas; Jonsson, Andreas; Ivansson, Daniel; Lövgren, Ann; Borth, Nicole.
Affiliation
  • Weinguny M; ACIB-Austrian Centre of Industrial Biotechnology, Graz, Austria.
  • Eisenhut P; Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria.
  • Klanert G; ACIB-Austrian Centre of Industrial Biotechnology, Graz, Austria.
  • Virgolini N; Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria.
  • Marx N; ACIB-Austrian Centre of Industrial Biotechnology, Graz, Austria.
  • Jonsson A; ACIB-Austrian Centre of Industrial Biotechnology, Graz, Austria.
  • Ivansson D; ACIB-Austrian Centre of Industrial Biotechnology, Graz, Austria.
  • Lövgren A; Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna, Austria.
  • Borth N; Cytiva, Uppsala, Sweden.
Biotechnol Bioeng ; 117(11): 3435-3447, 2020 11.
Article in En | MEDLINE | ID: mdl-32662873
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA Modification Methylases / DNA Methylation / Epigenesis, Genetic / Gene Knockdown Techniques Limits: Animals Language: En Journal: Biotechnol Bioeng Year: 2020 Document type: Article Affiliation country: Austria Country of publication: United States
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA Modification Methylases / DNA Methylation / Epigenesis, Genetic / Gene Knockdown Techniques Limits: Animals Language: En Journal: Biotechnol Bioeng Year: 2020 Document type: Article Affiliation country: Austria Country of publication: United States