TCR-Seq Identifies Distinct Repertoires of Distant-Metastatic and Nondistant-Metastatic Thyroid Tumors.
J Clin Endocrinol Metab
; 105(9)2020 09 01.
Article
in En
| MEDLINE
| ID: mdl-32674137
ABSTRACT
CONTEXT Malignant thyroid tumor with distant metastasis is associated with poor outcome. Early detection of distant metastasis is of great clinical importance. OBJECTIVE:
Thyroid tumor infiltrated with T cells can serve as a biomarker for monitoring metastasis.DESIGN:
A retrospective analysis was performed of patient clinical samples collected between 2012 to 2018, using T-cell receptor sequencing (TCR-seq) for clinical exploration.SETTING:
This study took place at Zhejiang Cancer Hospital. PATIENTS Sixty-eight patients with papillary thyroid cancer (PTC) (distinct metastatic status) and 21 patients with benign nodules were enrolled. All patients had not received any treatment before surgery. MAIN OUTCOMEMEASURE:
The characteristics of TCRß complementary-determining region 3 (CDR3) for each patient were determined by high-throughput sequencing.RESULTS:
The TCRß diversity of malignant tumors is significantly higher than benign nodules both in blood and tumor samples (Shannon index, blood, Pâ <â .01; tumor, Pâ <â .001). The malignant tumors with distant metastasis or invasiveness showed lower TCRß diversity than nonmetastasis (Shannon index, Pâ <â .01) or noninvasive (Shannon index, Pâ <â .01) malignant tumors. Analysis of the Morisita-Horn similarity index indicated significant TCRß repertoire similarity between tumor and blood in distant-metastatic patients (comparison with nonmetastasis, Pâ <â .05). According to the discrepancy of the CDR3 among patients with different clinicopathological status, the classifier was constructed to discriminate distant-metastatic individuals. A promising area under the curve value of 83.8% was obtained with the number of overlapping CDR3 clonotypes.CONCLUSION:
The availability and reliability of TCR-seq render it prospective to translate these intrinsic attributes into clinical practice for monitoring distant metastasis in PTC patients.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Thyroid Neoplasms
/
Lymphocytes, Tumor-Infiltrating
/
Genes, T-Cell Receptor beta
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Thyroid Cancer, Papillary
Type of study:
Observational_studies
/
Prognostic_studies
/
Risk_factors_studies
/
Screening_studies
Limits:
Adult
/
Aged
/
Aged80
/
Female
/
Humans
/
Male
/
Middle aged
Language:
En
Journal:
J Clin Endocrinol Metab
Year:
2020
Document type:
Article
Affiliation country:
China