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Dual SMAD inhibition and Wnt inhibition enable efficient and reproducible differentiations of induced pluripotent stem cells into retinal ganglion cells.
Chavali, Venkata R M; Haider, Naqi; Rathi, Sonika; Vrathasha, Vrathasha; Alapati, Teja; He, Jie; Gill, Kamaljot; Nikonov, Roman; Duong, Thu T; McDougald, Devin S; Nikonov, Sergei; O'Brien, Joan; Mills, Jason A.
Affiliation
  • Chavali VRM; Scheie Eye Institute, University of Pennsylvania Perelman School of Medicine, 133A Anatomy Chemistry Building, 3620 Hamilton Walk, Philadelphia, PA, 19104, USA. vchavali@pennmedicine.upenn.edu.
  • Haider N; Scheie Eye Institute, University of Pennsylvania Perelman School of Medicine, 133A Anatomy Chemistry Building, 3620 Hamilton Walk, Philadelphia, PA, 19104, USA.
  • Rathi S; Scheie Eye Institute, University of Pennsylvania Perelman School of Medicine, 133A Anatomy Chemistry Building, 3620 Hamilton Walk, Philadelphia, PA, 19104, USA.
  • Vrathasha V; Scheie Eye Institute, University of Pennsylvania Perelman School of Medicine, 133A Anatomy Chemistry Building, 3620 Hamilton Walk, Philadelphia, PA, 19104, USA.
  • Alapati T; Scheie Eye Institute, University of Pennsylvania Perelman School of Medicine, 133A Anatomy Chemistry Building, 3620 Hamilton Walk, Philadelphia, PA, 19104, USA.
  • He J; Scheie Eye Institute, University of Pennsylvania Perelman School of Medicine, 133A Anatomy Chemistry Building, 3620 Hamilton Walk, Philadelphia, PA, 19104, USA.
  • Gill K; F.M. Kirby Center for Molecular Ophthalmology and Center for Advanced Retinal and Ocular Therapeutics (CAROT), 309B Stellar Chance Laboratories, Philadelphia, PA, 19104, USA.
  • Nikonov R; F.M. Kirby Center for Molecular Ophthalmology and Center for Advanced Retinal and Ocular Therapeutics (CAROT), 309B Stellar Chance Laboratories, Philadelphia, PA, 19104, USA.
  • Duong TT; F.M. Kirby Center for Molecular Ophthalmology and Center for Advanced Retinal and Ocular Therapeutics (CAROT), 309B Stellar Chance Laboratories, Philadelphia, PA, 19104, USA.
  • McDougald DS; F.M. Kirby Center for Molecular Ophthalmology and Center for Advanced Retinal and Ocular Therapeutics (CAROT), 309B Stellar Chance Laboratories, Philadelphia, PA, 19104, USA.
  • Nikonov S; F.M. Kirby Center for Molecular Ophthalmology and Center for Advanced Retinal and Ocular Therapeutics (CAROT), 309B Stellar Chance Laboratories, Philadelphia, PA, 19104, USA.
  • O'Brien J; Scheie Eye Institute, University of Pennsylvania Perelman School of Medicine, 133A Anatomy Chemistry Building, 3620 Hamilton Walk, Philadelphia, PA, 19104, USA.
  • Mills JA; F.M. Kirby Center for Molecular Ophthalmology and Center for Advanced Retinal and Ocular Therapeutics (CAROT), 309B Stellar Chance Laboratories, Philadelphia, PA, 19104, USA. millsja@pennmedicine.upenn.edu.
Sci Rep ; 10(1): 11828, 2020 07 16.
Article in En | MEDLINE | ID: mdl-32678240
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Retinal Ganglion Cells / Cell Differentiation / Wnt Proteins / Smad Proteins / Induced Pluripotent Stem Cells Limits: Humans Language: En Journal: Sci Rep Year: 2020 Document type: Article Affiliation country: United States Country of publication: United kingdom
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Retinal Ganglion Cells / Cell Differentiation / Wnt Proteins / Smad Proteins / Induced Pluripotent Stem Cells Limits: Humans Language: En Journal: Sci Rep Year: 2020 Document type: Article Affiliation country: United States Country of publication: United kingdom