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Reversible Protein Conjugation on Live Cell Surfaces by Specific Recognition between Coiled-Coil Motifs of Natural Amino Acid Sequences.
Ahn, Joon Hyung; Kang, Sunah; Park, Sohyun; Song, Hojoon; Yun, Yaejin; Choi, Sejong; Chong, Seung-Eun; Cheon, Dae Hee; Chun, Dahyun; Oh, Jae Hoon; Nam, Sohee; Lee, Yan.
Affiliation
  • Ahn JH; Department of Chemistry, Seoul National University, Seoul 08826, Republic of Korea.
  • Kang S; Department of Chemistry, Seoul National University, Seoul 08826, Republic of Korea.
  • Park S; Department of Chemistry, Seoul National University, Seoul 08826, Republic of Korea.
  • Song H; Department of Chemistry, Seoul National University, Seoul 08826, Republic of Korea.
  • Yun Y; Department of Chemistry, Seoul National University, Seoul 08826, Republic of Korea.
  • Choi S; Department of Chemistry, Seoul National University, Seoul 08826, Republic of Korea.
  • Chong SE; Department of Chemistry, Seoul National University, Seoul 08826, Republic of Korea.
  • Cheon DH; Department of Chemistry, Seoul National University, Seoul 08826, Republic of Korea.
  • Chun D; Department of Chemistry, Seoul National University, Seoul 08826, Republic of Korea.
  • Oh JH; Department of Chemistry, Seoul National University, Seoul 08826, Republic of Korea.
  • Nam S; Department of Chemistry, Seoul National University, Seoul 08826, Republic of Korea.
  • Lee Y; Department of Chemistry, Seoul National University, Seoul 08826, Republic of Korea.
Biomacromolecules ; 21(9): 3539-3546, 2020 09 14.
Article in En | MEDLINE | ID: mdl-32678573
In this study, we propose a reversible covalent conjugation method for peptides, proteins, and even live cells based on specific recognition between natural amino acid sequences. Two heptad sequences can specifically recognize each other and induce the formation of a disulfide bond between cysteine residues. We show the covalent bond formation and dissociation between peptides and proteins in cell-free conditions and on the surface of live cells. Because heptad sequences consist of natural amino acids, they are expressed in cells without additional preparation and can be used to selectively conjugate peptides, proteins, and cells.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Peptides / Cysteine Language: En Journal: Biomacromolecules Journal subject: BIOLOGIA MOLECULAR Year: 2020 Document type: Article Country of publication: United States
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Peptides / Cysteine Language: En Journal: Biomacromolecules Journal subject: BIOLOGIA MOLECULAR Year: 2020 Document type: Article Country of publication: United States