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Molecular malaria surveillance using a novel protocol for extraction and analysis of nucleic acids retained on used rapid diagnostic tests.
Guirou, Etienne A; Schindler, Tobias; Hosch, Salome; Donfack, Olivier Tresor; Yoboue, Charlene Aya; Krähenbühl, Silvan; Deal, Anna; Cosi, Glenda; Gondwe, Linda; Mwangoka, Grace; Masuki, Heavenlight; Salim, Nahya; Mpina, Maxmillian; Said, Jongo; Abdulla, Salim; Hoffman, Stephen L; Nlavo, Bonifacio Manguire; Maas, Carl; Falla, Carlos Cortes; Phiri, Wonder P; Garcia, Guillermo A; Tanner, Marcel; Daubenberger, Claudia.
Affiliation
  • Guirou EA; Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, Basel, Switzerland.
  • Schindler T; University of Basel, Basel, Switzerland.
  • Hosch S; Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, Basel, Switzerland. tobias.schindler@swisstph.ch.
  • Donfack OT; University of Basel, Basel, Switzerland. tobias.schindler@swisstph.ch.
  • Yoboue CA; Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, Basel, Switzerland.
  • Krähenbühl S; University of Basel, Basel, Switzerland.
  • Deal A; Medical Care Development International, Malabo, Equatorial Guinea.
  • Cosi G; Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, Basel, Switzerland.
  • Gondwe L; University of Basel, Basel, Switzerland.
  • Mwangoka G; Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, Basel, Switzerland.
  • Masuki H; University of Basel, Basel, Switzerland.
  • Salim N; Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, Basel, Switzerland.
  • Mpina M; University of Basel, Basel, Switzerland.
  • Said J; Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, Basel, Switzerland.
  • Abdulla S; University of Basel, Basel, Switzerland.
  • Hoffman SL; Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, Basel, Switzerland.
  • Nlavo BM; University of Basel, Basel, Switzerland.
  • Maas C; Ifakara Health Institute, Bagamoyo Branch, United Republic of Tanzania.
  • Falla CC; Ifakara Health Institute, Bagamoyo Branch, United Republic of Tanzania.
  • Phiri WP; Department of Paediatrics and Child Health, Muhimbili University of Health and Allied Sciences, Dar Es Salaam, Tanzania.
  • Garcia GA; Department of Paediatrics and Child Health, Muhimbili University of Health and Allied Sciences, Dar Es Salaam, Tanzania.
  • Tanner M; Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, Basel, Switzerland.
  • Daubenberger C; University of Basel, Basel, Switzerland.
Sci Rep ; 10(1): 12305, 2020 07 23.
Article in En | MEDLINE | ID: mdl-32703999
The use of malaria rapid diagnostic tests (RDTs) as a source for nucleic acids that can be analyzed via nucleic acid amplification techniques has several advantages, including minimal amounts of blood, sample collection, simplified storage and shipping conditions at room temperature. We have systematically developed and extensively evaluated a procedure to extract total nucleic acids from used malaria RDTs. The co-extraction of DNA and RNA molecules from small volumes of dried blood retained on the RDTs allows detection and quantification of P. falciparum parasites from asymptomatic patients with parasite densities as low as 1 Pf/µL blood using reverse transcription quantitative PCR. Based on the extraction protocol we have developed the ENAR (Extraction of Nucleic Acids from RDTs) approach; a complete workflow for large-scale molecular malaria surveillance. Using RDTs collected during a malaria indicator survey we demonstrated that ENAR provides a powerful tool to analyze nucleic acids from thousands of RDTs in a standardized and high-throughput manner. We found several, known and new, non-synonymous single nucleotide polymorphisms in the propeller region of the kelch 13 gene among isolates circulating on Bioko Island, Equatorial Guinea.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Nucleic Acids / Malaria, Falciparum / Molecular Diagnostic Techniques Type of study: Diagnostic_studies / Guideline / Screening_studies Limits: Adult / Animals / Female / Humans / Male Country/Region as subject: Africa Language: En Journal: Sci Rep Year: 2020 Document type: Article Affiliation country: Switzerland Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Nucleic Acids / Malaria, Falciparum / Molecular Diagnostic Techniques Type of study: Diagnostic_studies / Guideline / Screening_studies Limits: Adult / Animals / Female / Humans / Male Country/Region as subject: Africa Language: En Journal: Sci Rep Year: 2020 Document type: Article Affiliation country: Switzerland Country of publication: United kingdom