Single molecule mass photometry of nucleic acids.
Nucleic Acids Res
; 48(17): e97, 2020 09 25.
Article
in En
| MEDLINE
| ID: mdl-32756898
ABSTRACT
Mass photometry is a recently developed methodology capable of measuring the mass of individual proteins under solution conditions. Here, we show that this approach is equally applicable to nucleic acids, enabling their facile, rapid and accurate detection and quantification using sub-picomoles of sample. The ability to count individual molecules directly measures relative concentrations in complex mixtures without need for separation. Using a dsDNA ladder, we find a linear relationship between the number of bases per molecule and the associated imaging contrast for up to 1200 bp, enabling us to quantify dsDNA length with up to 2 bp accuracy. These results introduce mass photometry as an accurate, rapid and label-free single molecule method complementary to existing DNA characterization techniques.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Photometry
/
Mass Spectrometry
/
DNA
/
Single Molecule Imaging
Language:
En
Journal:
Nucleic Acids Res
Year:
2020
Document type:
Article
Affiliation country:
United kingdom