Involvement of Aquaporins in the Intense Pulsed Light-Enhanced Wound Healing in Diabetic Rats.
Lasers Surg Med
; 53(4): 549-556, 2021 04.
Article
in En
| MEDLINE
| ID: mdl-32757279
ABSTRACT
BACKGROUND AND OBJECTIVES:
We previously demonstrated that intense pulsed light (IPL) irradiation prior to wounding improved the wound healing in rats with diabetes mellitus (DM). Also, we found that IPL upregulated the expression of aquaporin 3 (AQP3), a protein that is crucial for wound healing, in normal rats. This present study aimed to examine the involvement of AQPs in the IPL-enhanced wound healing in diabetic rats. STUDY DESIGN/MATERIALS ANDMETHODS:
Streptozotocin was used to induce diabetes in Sprague-Dawley rats. Animals were divided into four groups normal group, DM only group, DM rats with IPL treatment 2 weeks before wounding (DM + IPL-Pre group), and DM rats with concurrent IPL irradiation and wounding (DM + IPL-Con group). Wounds were created on the dorsal skin of rats. The expressions of AQP1, 3, 4, 7, and 9 in the pre-injured skin, periwound, and wound were determined.RESULTS:
Among all the AQPs analyzed, only the expressions of AQP3 and AQP7 were significantly altered. Unirradiated diabetic rats showed much higher expression level of AQP3 in the regenerating skin compared with normal rats. IPL pretreatment, but not concurrent treatment, attenuated the expression toward the level detected in the normal wounds. In contrast, a lower expression level of AQP7 was noted in the regenerating skin of DM only rats and IPL pretreatment upregulated the expression to a level similar to that in the normal rats.CONCLUSION:
The beneficial effect of IPL pretreatment on the wound healing in diabetic rats might involve a mechanism by which the expression of AQPs is regulated. Lasers Surg. Med. © 2020 Wiley Periodicals LLC.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Phototherapy
/
Wound Healing
/
Aquaporins
/
Diabetes Mellitus, Experimental
Limits:
Animals
Language:
En
Journal:
Lasers Surg Med
Year:
2021
Document type:
Article
Affiliation country:
Taiwan