Fusaric acid-induced epigenetic modulation of hepatic H3K9me3 triggers apoptosis in vitro and in vivo.
Epigenomics
; 12(11): 955-972, 2020 06.
Article
in En
| MEDLINE
| ID: mdl-32762452
ABSTRACT
Aim:
To determine the effect of the food-borne mycotoxin, fusaric acid (FA) on miR-200a, SUV39H1-mediated H3K9me3, genome integrity and apoptosis in human liver (HepG2) cells and C57BL/6 mice livers. Materials &methods:
MiR-200a, Sirt1, SUV39H1-mediated H3K9me3, genome integrity and apoptosis was measured in HepG2 cells and C57BL/6 mice livers using qPCR, western blot, DNA electrophoresis and luminometry.Results:
FA upregulated miR-200a and decreased Sirt1 expression in HepG2 cells and mice livers; decreased expression of SUV39H1 and KDM4B, thus decreasing H3K9me3 and increasing H3K9me1; increased cell mortality via apoptosis.Conclusion:
FA induced apoptosis by upregulating miR-200a and decreasing SUV39H1-mediated H3K9me3 in HepG2 cells and mice livers.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Fusaric Acid
/
Apoptosis
/
Epigenesis, Genetic
/
Liver
Limits:
Animals
/
Humans
/
Male
Language:
En
Journal:
Epigenomics
Year:
2020
Document type:
Article
Affiliation country:
South Africa