Combination effect of exercise training and eugenol supplementation on the hippocampus apoptosis induced by chlorpyrifos.
Mol Biol Rep
; 47(8): 5985-5996, 2020 Aug.
Article
in En
| MEDLINE
| ID: mdl-32780254
ABSTRACT
The aim of this study was to investigate the combination effect of exercise training and eugenol supplementation on the hippocampus apoptosis induced by CPF. 64 adult male albino rats were randomly selected and devided into eight groups of eight including control, exercise (EXE), chlorpyrifos (CPF), Control + Oil (Co + Oil), Control + DMSO (Co + DMSO), chlorpyrifos + eugenol (CPF + Sup), chlorpyrifos + exercise (CPF + Exe) and, chlorpyrifos + exercise + eugenol (CPF + Exe + Eu). Four experimental groups received intraperitoneal injection (5 days a week) of 3.0 mg/kg body weight CPF in DMSO for 6 consecutive weeks. The exercise groups performed aerobic 5 days per week over 4 weeks. Eugenol were administered by gavage. Finally, the animals were sacrificed using CO2 gas (a half of the rats were anesthetized with ketamine and xylazine and then perfused) to evaluate hippocampus histology and parameters. The results of this study showed that CPF injection significantly decreased BDNF, AChE and ATP in CA1 area of the hippocampus (p Ë 0.05). Also, CA1 apoptosis by tunnel assay, it was found that CPF receiving groups with different dosage, showed a significant increase compared to other groups, which was confirmed by increasing cytochrome C and procaspase-3 in CPF groups (p Ë 0.05). The result of this study show that 4 weeks of exercise training and eugenol supplementation does not improve the destructive effects of CPF in CA1 area of the hippocampus. As a result, it is recommended that future studies longer periods for treatment with exercise and eugenol supplementation.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Physical Conditioning, Animal
/
Eugenol
/
Apoptosis
/
Chlorpyrifos
/
Exercise Therapy
/
Organophosphate Poisoning
/
Hippocampus
Type of study:
Prognostic_studies
Limits:
Animals
Language:
En
Journal:
Mol Biol Rep
Year:
2020
Document type:
Article
Affiliation country:
Iran