Gamma radiation-induced grafting of n-hydroxyethyl acrylamide onto poly(3-hydroxybutyrate): A companion study on its polyurethane scaffolds meant for potential skin tissue engineering applications.
Mater Sci Eng C Mater Biol Appl
; 116: 111176, 2020 Nov.
Article
in En
| MEDLINE
| ID: mdl-32806310
ABSTRACT
This study aimed at investigating the synthesis, characterization, and search for a biotechnological application proposal for poly [(R)-3-hydroxybutyric acid] (PHB) grafted with the n-hydroxyethyl acrylamide (HEAA) monomer. The novel copolymer was prepared by 60Co gamma radiation-induced-graft polymerization. The effect of different solvents in the graft polymerization; the degree of grafting, crystallinity, and hydrophilicity; the morphology and the thermal properties were evaluated. The polyurethane fabricated from the grafted PHB was suggested as a scaffold. The enzymatic degradation behavior and the spectroscopic, morphological, mechanical, and biological properties of the composites were assessed. According to the results, the successful grafting of HEAA onto PHB was verified. The grafting was significantly affected by the type of solvent employed. A decreased crystallinity and increased hydrophilicity of the graft copolymer, concerning the PHB, was found. An increased roughness was observed in the morphology of the polymer after grafting. The thermodynamic parameters, except for the glass transition temperature, also decreased for the synthetic biopolymer. The intended use of these scaffolds for skin tissue engineering was supported by a proper degradability and degree of porosity, improved mechanical properties, the optimal culture of human fibroblasts, and its transfection with a plasmid vector containing an enhanced green fluorescent protein.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Polyurethanes
/
Tissue Engineering
Limits:
Humans
Language:
En
Journal:
Mater Sci Eng C Mater Biol Appl
Year:
2020
Document type:
Article
Affiliation country:
Mexico