Identification of Rho GEF and RhoA Activation by Pull-Down Assays.

Sajib, Md S; Zahra, Fatema T; Akwii, Racheal G; Mikelis, Constantinos M

Sajib, Md S; Zahra, Fatema T; Akwii, Racheal G; Mikelis, Constantinos M.

Affiliation

Sajib MS; Department of Pharmaceutical Sciences, Jerry H. Hodge School of Pharmacy, Texas Tech University Health Sciences Center, Amarillo, TX, USA.
Zahra FT; Department of Pharmaceutical Sciences, Jerry H. Hodge School of Pharmacy, Texas Tech University Health Sciences Center, Amarillo, TX, USA.
Akwii RG; Department of Pharmaceutical Sciences, Jerry H. Hodge School of Pharmacy, Texas Tech University Health Sciences Center, Amarillo, TX, USA.
Mikelis CM; Department of Pharmaceutical Sciences, Jerry H. Hodge School of Pharmacy, Texas Tech University Health Sciences Center, Amarillo, TX, USA. constantinos.mikelis@ttuhsc.edu.

Methods Mol Biol ; 2193: 97-109, 2021.

Article in En | MEDLINE | ID: mdl-32808262

Subject(s)

GTPase-Activating Proteins/genetics; Molecular Biology/methods; rhoA GTP-Binding Protein/genetics; GTPase-Activating Proteins/isolation & purification; Guanosine Diphosphate/genetics; Guanosine Triphosphate/genetics; Humans; Protein Binding/genetics; Rho Guanine Nucleotide Exchange Factors/genetics; Rho Guanine Nucleotide Exchange Factors/isolation & purification; rhoA GTP-Binding Protein/isolation & purification

Key words

GEF; GEF pull-down assay; RhoA; RhoA pull-down assay; Rhotekin

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: RhoA GTP-Binding Protein / GTPase-Activating Proteins / Molecular Biology Type of study: Diagnostic_studies / Prognostic_studies Limits: Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2021 Document type: Article Affiliation country: United States

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