Sensitive identification of prostate-specific antigen by iron oxide nanoparticle antibody conjugates on the gap-finger electrode surface.
Biotechnol Appl Biochem
; 68(4): 896-901, 2021 Aug.
Article
in En
| MEDLINE
| ID: mdl-32822079
ABSTRACT
Researches have proved that increasing level of prostate-specific antigen (PSA) is an indicator for the progression of prostate cancer. The present study was focused to determine the PSA level by using anti-PSA antibody conjugated iron oxide nanoparticles, as the probe immobilized on the gap-fingered electrode sensing surface. The detection limit and sensitivity were found at the level of 1.9 pg/mL on the linear regression curve (y = 1.6939x - 0.5671; R² = 0.9878). A dose-dependent liner range was found from 1.9 until 60 pg/mL. Further, PSA was spiked in human serum and did not affect the interaction of PSA and its antibody. This method of detection quantifies the level of PSA, which helps to diagnose prostate cancer at its earlier stage.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Prostatic Neoplasms
/
Biomarkers, Tumor
/
Prostate-Specific Antigen
/
Magnetic Iron Oxide Nanoparticles
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Humans
/
Male
Language:
En
Journal:
Biotechnol Appl Biochem
Journal subject:
BIOQUIMICA
/
BIOTECNOLOGIA
Year:
2021
Document type:
Article
Affiliation country:
China