PhosphoShield: Improving Trypsin Digestion of Phosphoproteins by Shielding the Negatively Charged Phosphate Moiety.

Bubis, Julia A; Gorshkov, Vladimir; Gorshkov, Mikhail V; Kjeldsen, Frank

Bubis, Julia A; Gorshkov, Vladimir; Gorshkov, Mikhail V; Kjeldsen, Frank.

Affiliation

Bubis JA; V. L. Talrose Institute for Energy Problems of Chemical Physics, N. N. Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, 119334 Moscow, Russia.
Gorshkov V; Moscow Institute of Physics and Technology (State University), 141700 Dolgoprudny, Russia.
Gorshkov MV; Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230 Odense M, Denmark.
Kjeldsen F; Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230 Odense M, Denmark.

J Am Soc Mass Spectrom ; 31(10): 2053-2060, 2020 Oct 07.

Article in En | MEDLINE | ID: mdl-32840367

Subject(s)

Phosphopeptides/analysis; Phosphoproteins/chemistry; Tandem Mass Spectrometry/methods; Hep G2 Cells; Humans; Models, Molecular; Phosphates/chemistry; Protein Processing, Post-Translational; Proteolysis; Proteomics/methods; Trypsin/chemistry

Key words

digallium complex; metal affinity; missed cleavages; phosphoproteomics; sample preparation; trypsin digestion

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Phosphopeptides / Phosphoproteins / Tandem Mass Spectrometry Limits: Humans Language: En Journal: J Am Soc Mass Spectrom Year: 2020 Document type: Article Affiliation country: RUSSIA Country of publication: United States

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