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EPR Spectroscopy as a Method for ROS Quantification in the Skin.
Lohan, Silke B; Ivanov, Daniela; Schüler, Nadine; Berger, Bastian; Albrecht, Stephanie; Meinke, Martina C.
Affiliation
  • Lohan SB; Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Department of Dermatology, Venerology and Allergology, Center of Experimental and Applied Cutaneous Physiology, Berlin, Germany.
  • Ivanov D; Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Department of Dermatology, Venerology and Allergology, Center of Experimental and Applied Cutaneous Physiology, Berlin, Germany.
  • Schüler N; Freiberg Instruments GmbH, Freiberg, Germany.
  • Berger B; Freiberg Instruments GmbH, Freiberg, Germany.
  • Albrecht S; Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Department of Dermatology, Venerology and Allergology, Center of Experimental and Applied Cutaneous Physiology, Berlin, Germany.
  • Meinke MC; Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institute of Health, Department of Dermatology, Venerology and Allergology, Center of Experimental and Applied Cutaneous Physiology, Berlin, Germany. Martina.meinke@charite.
Methods Mol Biol ; 2202: 137-148, 2021.
Article in En | MEDLINE | ID: mdl-32857353
ABSTRACT
Electron paramagnetic resonance (EPR) spectroscopy is an established method for the measurement of free radicals. Solar radiation is essential for human life as it stimulates vitamin D synthesis and well-being. However, an excessive dose of solar radiation leads to the formation of free radicals. Here, we describe an EPR method for measuring the amount of radicals induced by UVA irradiation in excised skin. For the first time, a wavelength stable UVA LED (365 nm) was used. The method allows the quantitative determination of radicals in skin before, during, and after UVA irradiation. A dose-dependent radical production could be demonstrated, independent of the yielded power.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Skin / Reactive Oxygen Species / Electron Spin Resonance Spectroscopy Limits: Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2021 Document type: Article Affiliation country: Germany
Fulltext
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Skin / Reactive Oxygen Species / Electron Spin Resonance Spectroscopy Limits: Humans Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2021 Document type: Article Affiliation country: Germany