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Surveillance of cohesin-supported chromosome structure controls meiotic progression.
Castellano-Pozo, Maikel; Pacheco, Sarai; Sioutas, Georgios; Jaso-Tamame, Angel Luis; Dore, Marian H; Karimi, Mohammad M; Martinez-Perez, Enrique.
Affiliation
  • Castellano-Pozo M; MRC London Institute of Medical Sciences, London, W12 0NN, UK.
  • Pacheco S; MRC London Institute of Medical Sciences, London, W12 0NN, UK.
  • Sioutas G; MRC London Institute of Medical Sciences, London, W12 0NN, UK.
  • Jaso-Tamame AL; MRC London Institute of Medical Sciences, London, W12 0NN, UK.
  • Dore MH; MRC London Institute of Medical Sciences, London, W12 0NN, UK.
  • Karimi MM; MRC London Institute of Medical Sciences, London, W12 0NN, UK.
  • Martinez-Perez E; MRC London Institute of Medical Sciences, London, W12 0NN, UK. fadri@imperial.ac.uk.
Nat Commun ; 11(1): 4345, 2020 08 28.
Article in En | MEDLINE | ID: mdl-32859945
Chromosome movements and programmed DNA double-strand breaks (DSBs) promote homologue pairing and initiate recombination at meiosis onset. Meiotic progression involves checkpoint-controlled termination of these events when all homologue pairs achieve synapsis and form crossover precursors. Exploiting the temporo-spatial organisation of the C. elegans germline and time-resolved methods of protein removal, we show that surveillance of the synaptonemal complex (SC) controls meiotic progression. In nuclei with fully synapsed homologues and crossover precursors, removing different meiosis-specific cohesin complexes, which are individually required for SC stability, or a SC central region component causes functional redeployment of the chromosome movement and DSB machinery, triggering whole-nucleus reorganisation. This apparent reversal of the meiotic programme requires CHK-2 kinase reactivation via signalling from chromosome axes containing HORMA proteins, but occurs in the absence of transcriptional changes. Our results uncover an unexpected plasticity of the meiotic programme and show how chromosome signalling orchestrates nuclear organisation and meiotic progression.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Chromosomal Proteins, Non-Histone / Caenorhabditis elegans / Cell Cycle Proteins / Chromosome Structures / Meiosis Type of study: Screening_studies Limits: Animals Language: En Journal: Nat Commun Journal subject: BIOLOGIA / CIENCIA Year: 2020 Document type: Article Country of publication: United kingdom

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Chromosomal Proteins, Non-Histone / Caenorhabditis elegans / Cell Cycle Proteins / Chromosome Structures / Meiosis Type of study: Screening_studies Limits: Animals Language: En Journal: Nat Commun Journal subject: BIOLOGIA / CIENCIA Year: 2020 Document type: Article Country of publication: United kingdom