Rbm24 regulates inner-ear-specific alternative splicing and is essential for maintaining auditory and motor coordination.
RNA Biol
; 18(4): 468-480, 2021 04.
Article
in En
| MEDLINE
| ID: mdl-32887533
ABSTRACT
Tissue-specific alternative splicing (AS) is emerging as one of the most exciting types of mechanisms associated with organ development and disease. In the auditory system, many hearing-related genes undergo AS, and errors in this process result in syndromic or non-syndromic hearing loss. However, little is known about the factors and mechanisms directing AS in the inner ear. In the present study, we identified a novel RNA-binding protein, Rbm24, which was critically involved in regulating inner-ear-specific AS. Rbm24 deletion resulted in hearing loss and defects in motor coordination. Global splicing analysis showed Rbm24 was required for correct splicing of a subset of pre-mRNA transcripts with essential roles in stereocilia integrity and survival of hair cells. Furthermore, we identified that Rbm24 directly regulated the splicing of Cdh23, a known disease gene responsible for human Usher syndrome 1D and non-syndromic autosomal recessive deafness DFNB12. In conclusion, our findings demonstrated that Rbm24 was a critical factor in regulating inner-ear-specific splicing and maintaining the hearing and motor coordination function of the inner ear. Our data not only offer mechanistic insights but also provide functional annotation of Rbm24 splicing targets that contribute to hearing loss.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Psychomotor Performance
/
RNA-Binding Proteins
/
Alternative Splicing
/
Ear, Inner
Type of study:
Prognostic_studies
Limits:
Animals
/
Humans
Language:
En
Journal:
RNA Biol
Journal subject:
BIOLOGIA MOLECULAR
Year:
2021
Document type:
Article
Affiliation country:
China