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An expanded palette of dopamine sensors for multiplex imaging in vivo.
Patriarchi, Tommaso; Mohebi, Ali; Sun, Junqing; Marley, Aaron; Liang, Ruqiang; Dong, Chunyang; Puhger, Kyle; Mizuno, Grace Or; Davis, Carolyn M; Wiltgen, Brian; von Zastrow, Mark; Berke, Joshua D; Tian, Lin.
Affiliation
  • Patriarchi T; Department of Biochemistry and Molecular Medicine, University of California, Davis, Sacramento, CA, USA.
  • Mohebi A; Institute of Pharmacology and Toxicology, University of Zurich, Zurich, Switzerland.
  • Sun J; Neuroscience Center Zurich, University and ETH Zurich, Zurich, Switzerland.
  • Marley A; Department of Neurology, University of California, San Francisco, San Francisco, CA, USA.
  • Liang R; Department of Biochemistry and Molecular Medicine, University of California, Davis, Sacramento, CA, USA.
  • Dong C; Center for Neuroscience, University of California, Davis, Davis, CA, USA.
  • Puhger K; Key Laboratory of Medical Electrophysiology, Institute of Cardiovascular Research, Southwest Medical University, Luzhou, China.
  • Mizuno GO; Department of Psychiatry, University of California, San Francisco, San Francisco, CA, USA.
  • Davis CM; Department of Biochemistry and Molecular Medicine, University of California, Davis, Sacramento, CA, USA.
  • Wiltgen B; Department of Biochemistry and Molecular Medicine, University of California, Davis, Sacramento, CA, USA.
  • von Zastrow M; Center for Neuroscience, University of California, Davis, Davis, CA, USA.
  • Berke JD; Department of Biochemistry and Molecular Medicine, University of California, Davis, Sacramento, CA, USA.
  • Tian L; Department of Biochemistry and Molecular Medicine, University of California, Davis, Sacramento, CA, USA.
Nat Methods ; 17(11): 1147-1155, 2020 11.
Article in En | MEDLINE | ID: mdl-32895537
Genetically encoded dopamine sensors based on green fluorescent protein (GFP) enable high-resolution imaging of dopamine dynamics in behaving animals. However, these GFP-based variants cannot be readily combined with commonly used optical sensors and actuators, due to spectral overlap. We therefore engineered red-shifted variants of dopamine sensors called RdLight1, based on mApple. RdLight1 can be combined with GFP-based sensors with minimal interference and shows high photostability, permitting prolonged continuous imaging. We demonstrate the utility of RdLight1 for receptor-specific pharmacological analysis in cell culture, simultaneous assessment of dopamine release and cell-type-specific neuronal activity and simultaneous subsecond monitoring of multiple neurotransmitters in freely behaving rats. Dual-color photometry revealed that dopamine release in the nucleus accumbens evoked by reward-predictive cues is accompanied by a rapid suppression of glutamate release. By enabling multiplexed imaging of dopamine with other circuit components in vivo, RdLight1 opens avenues for understanding many aspects of dopamine biology.
Subject(s)

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Behavior, Animal / Brain / Biosensing Techniques / Dopamine / Neurons Limits: Animals / Humans Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2020 Document type: Article Affiliation country: United States Country of publication: United States

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Behavior, Animal / Brain / Biosensing Techniques / Dopamine / Neurons Limits: Animals / Humans Language: En Journal: Nat Methods Journal subject: TECNICAS E PROCEDIMENTOS DE LABORATORIO Year: 2020 Document type: Article Affiliation country: United States Country of publication: United States