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A Multichannel Pattern-Recognition-Based Protein Sensor with a Fluorophore-Conjugated Single-Stranded DNA Set.
Okada, Mari; Sugai, Hiroka; Tomita, Shunsuke; Kurita, Ryoji.
Affiliation
  • Okada M; Health and Medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
  • Sugai H; Faculty of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8573, Japan.
  • Tomita S; Health and Medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
  • Kurita R; Health and Medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan.
Sensors (Basel) ; 20(18)2020 Sep 08.
Article in En | MEDLINE | ID: mdl-32911729
Recently, pattern-recognition-based protein sensing has received considerable attention because it offers unique opportunities that complement more conventional antibody-based detection methods. Here, we report a multichannel pattern-recognition-based sensor using a set of fluorophore-conjugated single-stranded DNAs (ssDNAs), which can detect various proteins. Three different fluorophore-conjugated ssDNAs were placed into a single microplate well together with a target protein, and the generated optical response pattern that corresponds to each environment-sensitive fluorophore was read via multiple detection channels. Multivariate analysis of the resulting optical response patterns allowed an accurate detection of eight different proteases, indicating that fluorescence signal acquisition from a single compartment containing a mixture of ssDNAs is an effective strategy for the characterization of the target proteins. Additionally, the sensor could identify proteins, which are potential targets for disease diagnosis, in a protease and inhibitor mixture of different composition ratios. As our sensor benefits from simple construction and measurement procedures, and uses accessible materials, it offers a rapid and simple platform for the detection of proteins.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA, Single-Stranded / Fluorescent Dyes Language: En Journal: Sensors (Basel) Year: 2020 Document type: Article Affiliation country: Japan Country of publication: Switzerland

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: DNA, Single-Stranded / Fluorescent Dyes Language: En Journal: Sensors (Basel) Year: 2020 Document type: Article Affiliation country: Japan Country of publication: Switzerland