Osteoprotective effect of green tea polyphenols and annatto-extracted tocotrienol in obese mice is associated with enhanced microbiome vitamin K2 biosynthetic pathways.
J Nutr Biochem
; 86: 108492, 2020 12.
Article
in En
| MEDLINE
| ID: mdl-32920088
ABSTRACT
The role of the gut microbiome in bone health has received significant attention in the past decade. We investigated the effects of green tea polyphenols (GTP) and annatto-extracted tocotrienols (AT) on bone properties and gut microbiome in obese mice. Male mice were assigned to a two (no AT vs. 400 mg/kg diet AT)â¯×â¯two (no GTP vs. 0.5% w/v GTP) factorial design, namely control, G, T, and G+T group respectively, for 14 weeks. The 4th lumbar vertebra (LV-4) and femur were harvested for bone microstructural analysis using µ-CT. Microbiome analysis using 16S rRNA gene sequencing of cecal feces was performed. AT increased bone volume at distal femur. GTP increased serum procollagen type 1 N-terminal propeptide concentration, bone volume at the distal femur and the LV-4, and trabecular number at distal femur; whereas GTP decreased trabecular separation at distal femur. Interactions between GTP and AT were observed in serum C-terminal telopeptide of type I collagen level (control>G=T=G+T) as well as the cortical bone area (controlKey words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Tea
/
Bone and Bones
/
Plant Extracts
/
Carotenoids
/
Vitamin K 2
/
Tocotrienols
/
Bixaceae
/
Polyphenols
/
Microbiota
Type of study:
Risk_factors_studies
Limits:
Animals
Language:
En
Journal:
J Nutr Biochem
Journal subject:
BIOQUIMICA
/
CIENCIAS DA NUTRICAO
Year:
2020
Document type:
Article