Quantitative Protein Corona Composition and Dynamics on Carbon Nanotubes in Biological Environments.
Angew Chem Int Ed Engl
; 59(52): 23668-23677, 2020 12 21.
Article
in En
| MEDLINE
| ID: mdl-32931615
ABSTRACT
When nanoparticles enter biological environments, proteins adsorb to form the "protein corona" which alters nanoparticle biodistribution and toxicity. Herein, we measure protein corona formation on DNA-functionalized single-walled carbon nanotubes (ssDNA-SWCNTs), a nanoparticle used widely for sensing and delivery, in blood plasma and cerebrospinal fluid. We characterize corona composition by mass spectrometry, revealing high-abundance corona proteins involved in lipid binding, complement activation, and coagulation. We investigate roles of electrostatic and entropic interactions driving selective corona formation. Lastly, we study real-time protein binding on ssDNA-SWCNTs, obtaining agreement between enriched proteins binding strongly and depleted proteins binding marginally, while highlighting cooperative adsorption mechanisms. Knowledge of protein corona composition, formation mechanisms, and dynamics informs nanoparticle translation from in vitro design to in vivo application.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Nanotechnology
/
Nanotubes, Carbon
/
Nanoparticles
/
Protein Corona
Limits:
Humans
Language:
En
Journal:
Angew Chem Int Ed Engl
Year:
2020
Document type:
Article
Affiliation country:
United States