Cryptosporidium parvum - zoonotic subtype IIdA15G1 in a Slovakian patient.
Ann Agric Environ Med
; 27(3): 485-488, 2020 Sep 11.
Article
in En
| MEDLINE
| ID: mdl-32955234
ABSTRACT
INTRODUCTION AND OBJECTIVES:
The parasite Cryptosporidium spp. is an intracellular protozoa which has a broad range of hosts and zoonotic potential. It presents a serious health risk for agricultural workers and veterinarians. The aim of the study was to identify the species and subtypes of Cryptosporidium occurring in a veterinary student who came into contact with calves on a farm. MATERIAL ANDMETHODS:
The Ziehl-Neelsen staining technique was employed to confirm the presence of Cryptosporidium oocysts. ELISA test was applied to detect coproantigen in faecal specimens. Nested PCR was used to amplify a small ribosomal subunit (SSU rRNA) and sequencing of the GP60 gene served to identify the zoonotic subtypes.RESULTS:
The nested PCR allowed to confirm the C. parvum species; subsequently, the IIdA15G1 zoonotic subtype was identified.CONCLUSIONS:
This is the first confirmed case in Slovakia of human cryptosporidiosis caused by the unique subtype IIdA15G1.Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Cryptosporidium parvum
/
Cryptosporidiosis
Type of study:
Diagnostic_studies
/
Prognostic_studies
Limits:
Adult
/
Animals
/
Humans
Country/Region as subject:
Europa
Language:
En
Journal:
Ann Agric Environ Med
Journal subject:
SAUDE AMBIENTAL
Year:
2020
Document type:
Article