[Knockdown of choline kinase α (CHKA) inhibits the proliferation, invasion and migration of human U87MG glioma cells].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
; 36(8): 724-728, 2020 Aug.
Article
in Zh
| MEDLINE
| ID: mdl-32958130
ABSTRACT
Objective To detect the expression of choline kinase α (CHKA) in glioma and to further explore the effect of CHKA knockdown on the proliferation, invasion and migration of U87MG human glioma cells. Methods The mRNA expression of CHKA in high-grade gliomas and traumatic brain tissues was detected by real-time quantitative PCR. The expression of CHKA protein in high-grade gliomas and traumatic brain tissues was detected by Western blot analysis. The short hairpin RNA of CHKA (shCHKA) lentivirus and its control lentivirus (shNC) were constructed and used to infect U87MG glioma cells, which were then divided into the following three groups shCHKA group, shNC group and blank control group. The proliferation of U87MG cells was examined by CCK-8 assay, the invasion ability of glioma cells was tested by TranswellTM invasion assay, and the migration ability of glioma cells was evaluated by scratch healing test. Results The CHKA mRNA and protein were highly expressed in glioma. Knockdown of CHKA gene inhibited the proliferation, invasion and migration of U87MG glioma cells. Conclusion The expression of CHKA in glioma tissue is significantly higher than that in the normal brain tissue, and knockdown of CHKA gene inhibits the proliferation, invasion and migration of glioma cells. It suggests that CHKA may be related to the occurrence and development of gliomas.
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Collection:
01-internacional
Database:
MEDLINE
Main subject:
Brain Neoplasms
/
Choline Kinase
/
Glioma
/
Neoplasm Invasiveness
Limits:
Humans
Language:
Zh
Journal:
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
Journal subject:
ALERGIA E IMUNOLOGIA
Year:
2020
Document type:
Article
Affiliation country:
China