A new genre of fluorescence recovery assay to evaluate polo-like kinase 1 ATP-competitive inhibitors.
Anal Methods
; 12(36): 4418-4421, 2020 09 28.
Article
in En
| MEDLINE
| ID: mdl-32970049
ABSTRACT
Using a probe consisting of a fluorescein-labeled variant of the potent polo-like kinase 1 (Plk1) inhibitor BI2536 [FITC-PEG-Lys(BI2536) 4], we were able to determine half maximal inhibitory concentration (IC50) of ATP-competitive Type 1 inhibitors of Plk1 by means of a fluorescence recovery assay. This methodology represents a cost-effective and simple alternative to traditional kinase assays for initial screening of potential Plk1 inhibitors.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Protein Serine-Threonine Kinases
/
Cell Cycle Proteins
Language:
En
Journal:
Anal Methods
Year:
2020
Document type:
Article