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Role of PrPC in Cancer Stem Cell Characteristics and Drug Resistance in Colon Cancer Cells.
Go, Gyeongyun; Yun, Chul Won; Yoon, Yeo Min; Lim, Ji Ho; Lee, Jun Hee; Lee, Sang Hun.
Affiliation
  • Go G; Department of Biochemistry, Soonchunhyang University College of Medicine, Cheonan, Republic of Korea.
  • Yun CW; Medical Science Research Institute, Soonchunhyang University Seoul Hospital, Seoul, Republic of Korea.
  • Yoon YM; Medical Science Research Institute, Soonchunhyang University Seoul Hospital, Seoul, Republic of Korea.
  • Lim JH; Medical Science Research Institute, Soonchunhyang University Seoul Hospital, Seoul, Republic of Korea.
  • Lee JH; Department of Biochemistry, Soonchunhyang University College of Medicine, Cheonan, Republic of Korea.
  • Lee SH; College of Science and Technology, Dankook University, Cheonan, Republic of Korea.
Anticancer Res ; 40(10): 5611-5620, 2020 Oct.
Article in En | MEDLINE | ID: mdl-32988885
ABSTRACT
BACKGROUND/

AIM:

Cancer stem cell characteristics and drug resistance of colorectal cancer are associated with failure of cancer treatment. In this study, we investigated the effects of PrPC on cancer stem cell characteristics, migration, invasion, and drug resistance of 5FU-resistant CRC cells. MATERIALS AND

METHODS:

PrPC negative and PrPC positive cells were isolated from 5FU-resistant CRC cells using magnetic activated cell sorting. Sphere formation, cancer stem cell marker expression, migration, invasion, and drug resistance were analyzed.

RESULTS:

PrPC positive cells showed increased sphere formation capacity and increased expression of cancer stem cell markers compared to PrPC negative cells. In addition, PrPC positive cells showed increased migration, invasion and drug resistance compared to PrPC negative cells. Furthermore, knockdown of PrPC abolished these effects.

CONCLUSION:

PrPC expression is important in CRC cell behavior, such as sphere formation, migration, invasion, and drug resistance. PrPC is an important therapeutic target for the treatment of CRC.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Colonic Neoplasms / Drug Resistance, Neoplasm / Fluorouracil / Prion Proteins Limits: Humans Language: En Journal: Anticancer Res Year: 2020 Document type: Article
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Colonic Neoplasms / Drug Resistance, Neoplasm / Fluorouracil / Prion Proteins Limits: Humans Language: En Journal: Anticancer Res Year: 2020 Document type: Article