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Identification of a delayed leaf greening gene from a mutation of pummelo.
Yu, Hui-Wen; Lu, Zhi-Hao; Wang, Xia; Liu, Dan; He, Jia-Xian; Jiang, Xiao-Lin; Ke, Ling-Jun; Guo, Wen-Wu; Deng, Xiu-Xin; Xu, Qiang.
Affiliation
  • Yu HW; Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan, 430070, China.
  • Lu ZH; Key Laboratory of Landscape Plants with Fujian and Taiwan Characteristics of Fujian Colleges and Universities, Minnan Normal University, Zhangzhou, 363000, China.
  • Wang X; Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan, 430070, China.
  • Liu D; Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan, 430070, China.
  • He JX; Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan, 430070, China.
  • Jiang XL; Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan, 430070, China.
  • Ke LJ; Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan, 430070, China.
  • Guo WW; Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan, 430070, China.
  • Deng XX; Key Laboratory of Landscape Plants with Fujian and Taiwan Characteristics of Fujian Colleges and Universities, Minnan Normal University, Zhangzhou, 363000, China.
  • Xu Q; Key Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan, 430070, China.
Sci China Life Sci ; 64(7): 1165-1173, 2021 Jul.
Article in En | MEDLINE | ID: mdl-33009992
Delayed greening of young leaves is an unusual phenomenon of plants in nature. Citrus are mostly evergreen tree species. Here, a natural mutant of "Guanxi" pummelo (Citrus maxima), which shows yellow leaves at the young stage, was characterized to identify the genes underlying the trait of delayed leaf greening in plants. A segregating population with this mutant as the seed parent and a normal genotype as the pollen parent was generated. Two DNA pools respectively from the leaves of segregating seedlings with extreme phenotypes of normal leaf greening and delayed leaf greening were collected for sequencing. Bulked segregant analysis (BSA) and InDel marker analysis demonstrated that the delayed leaf greening trait is governed by a 0.3 Mb candidate region on chromosome 6. Gene expression analysis further identified a key candidate gene (Citrus Delayed Greening gene 1, CDG1) in the 0.3 Mb region, which showed significantly differential expression between the genotypes with delayed and normal leaf greening phenotypes. There was a 67 bp InDel region difference in the CDG1 promoter and the InDel region contains a TATA-box element. Confocal laser-scanning microscopy revealed that the CDG1-GFP fusion protein signals were co-localized with the chloroplast signals in the protoplasts. Overexpression of CDG1 in tobacco and Arabidopsis led to the phenotype of delayed leaf greening. These results suggest that the CDG1 gene is involved in controlling the delayed leaf greening phenotype with important functions in chloroplast development.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Protein Kinases / Citrus / Plant Leaves / Chloroplast Proteins Type of study: Diagnostic_studies Language: En Journal: Sci China Life Sci Journal subject: BIOLOGIA / CIENCIA Year: 2021 Document type: Article Affiliation country: China Country of publication: China

Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Protein Kinases / Citrus / Plant Leaves / Chloroplast Proteins Type of study: Diagnostic_studies Language: En Journal: Sci China Life Sci Journal subject: BIOLOGIA / CIENCIA Year: 2021 Document type: Article Affiliation country: China Country of publication: China