Co-immobilization of amylases in porous crosslinked gelatin matrices by different reticulations approaches.
Int J Biol Macromol
; 165(Pt A): 1002-1009, 2020 Dec 15.
Article
in En
| MEDLINE
| ID: mdl-33011269
ABSTRACT
The aim of this study was to carry out the co-immobilization of α-amylase and glucoamylase in crosslinked gelatin porous supports. For this, two methods of co-immobilization were proposed based on the crosslinking with glutaraldehyde (Ggta) or CaCl2 in presence of alginate (Gcal). The supports characterization revealed a porous microstructure with good interaction between its components according to the FTIR analysis and thermal properties. Optimal pH and temperature of the Gcal co-immobilized enzymes were determined at 60 °C and pH 6.0, present an enzymatic activity of 120 µmol·mL·min-1. Moreover, both supports were reused for up to 8 hydrolysis cycles. In addition, co-immobilized enzymes were more efficient than free enzymes in starch saccharification of starch in the long term. These results reveal that the co-immobilization of amylases in gelatinous supports is a promising approach in enzymatic chain reactions.
Key words
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
Starch
/
Enzymes, Immobilized
/
Alpha-Amylases
/
Gelatin
Language:
En
Journal:
Int J Biol Macromol
Year:
2020
Document type:
Article
Affiliation country:
Brazil