CRISPRoff enables spatio-temporal control of CRISPR editing.
Nat Commun
; 11(1): 5041, 2020 10 07.
Article
in En
| MEDLINE
| ID: mdl-33028827
ABSTRACT
Following introduction of CRISPR-Cas9 components into a cell, genome editing occurs unabated until degradation of its component nucleic acids and proteins by cellular processes. This uncontrolled reaction can lead to unintended consequences including off-target editing and chromosomal translocations. To address this, we develop a method for light-induced degradation of sgRNA termed CRISPRoff. Here we show that light-induced inactivation of ribonucleoprotein attenuates genome editing within cells and allows for titratable levels of editing efficiency and spatial patterning via selective illumination.
Full text:
1
Collection:
01-internacional
Database:
MEDLINE
Main subject:
RNA, Guide, Kinetoplastida
/
RNA Stability
/
CRISPR-Cas Systems
/
Gene Editing
/
Light
Limits:
Humans
Language:
En
Journal:
Nat Commun
Journal subject:
BIOLOGIA
/
CIENCIA
Year:
2020
Document type:
Article
Affiliation country:
United States