Establishment and validation of an in vitro co-culture model for oral cell lines using human PBMC-derived osteoclasts, osteoblasts, fibroblasts and keratinocytes.

Steller, Daniel; Scheibert, Alexandra; Sturmheit, Tabea; Hakim, Samer G

Steller, Daniel; Scheibert, Alexandra; Sturmheit, Tabea; Hakim, Samer G.

Affiliation

Steller D; Department of Maxillofacial Surgery, University Hospital of Lübeck, Ratzeburger Allee 160, 23562, Lübeck, Germany. Daniel.steller@uksh.de.
Scheibert A; Department of Maxillofacial Surgery, University Hospital of Lübeck, Ratzeburger Allee 160, 23562, Lübeck, Germany.
Sturmheit T; Fraunhofer Research Institution for Marine Biotechnology and Cell Technology, Mönkhofer Weg 239 a, 23562, Lübeck, Germany.
Hakim SG; Department of Maxillofacial Surgery, University Hospital of Lübeck, Ratzeburger Allee 160, 23562, Lübeck, Germany.

Sci Rep ; 10(1): 16861, 2020 10 08.

Article in En | MEDLINE | ID: mdl-33033302

Subject(s)

Bone Remodeling/physiology; Coculture Techniques/methods; Fibroblasts/physiology; Keratinocytes/physiology; Macrophage Colony-Stimulating Factor/pharmacology; Mouth/cytology; Osteoblasts/physiology; Osteoclasts/physiology; RANK Ligand/pharmacology; Bone Remodeling/drug effects; Cell Differentiation/drug effects; Cell Line; Cell Proliferation/drug effects; Cell Survival/drug effects; Culture Media; Humans

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Osteoblasts / Osteoclasts / Keratinocytes / Macrophage Colony-Stimulating Factor / Bone Remodeling / Coculture Techniques / RANK Ligand / Fibroblasts / Mouth Type of study: Prognostic_studies Limits: Humans Language: En Journal: Sci Rep Year: 2020 Document type: Article Affiliation country: Germany Country of publication: United kingdom

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