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Co-nanoencapsulated meloxicam and curcumin improves cognitive impairment induced by amyloid-beta through modulation of cyclooxygenase-2 in mice.
Gutierrez, Maria Eduarda Ziani; Savall, Anne Suély Pinto; da Luz Abreu, Edina; Nakama, Kelly Ayumi; Dos Santos, Renata Bem; Guedes, Marina Costa Monteiro; Ávila, Daiana Silva; Luchese, Cristiane; Haas, Sandra Elisa; Quines, Caroline Brandão; Pinton, Simone.
Affiliation
  • Gutierrez MEZ; Postgraduation Program in Biochemistry, Federal University of Pampa (UNIPAMPA), Uruguaiana, RS, Brazil.
  • Savall ASP; Postgraduation Program in Biochemistry, Federal University of Pampa (UNIPAMPA), Uruguaiana, RS, Brazil.
  • da Luz Abreu E; Postgraduation Program in Biochemistry, Federal University of Pampa (UNIPAMPA), Uruguaiana, RS, Brazil.
  • Nakama KA; Postgraduation Program in Pharmaceutical Science, Federal University of Pampa (UNIPAMPA), Uruguaiana, RS, Brazil.
  • Dos Santos RB; Postgraduation Program in Pharmaceutical Science, Federal University of Pampa (UNIPAMPA), Uruguaiana, RS, Brazil.
  • Guedes MCM; Postgraduation Program in Biochemistry, Federal University of Pampa (UNIPAMPA), Uruguaiana, RS, Brazil.
  • Ávila DS; Postgraduation Program in Biochemistry, Federal University of Pampa (UNIPAMPA), Uruguaiana, RS, Brazil.
  • Luchese C; Postgraduation Program in Biochemistry and Bioprospecting, Federal University of Pelotas (UFPEL), Capão do Leão, RS, Brazil.
  • Haas SE; Postgraduation Program in Biochemistry; Postgraduation Program in Pharmaceutical Science, Federal University of Pampa (UNIPAMPA), Uruguaiana, RS, Brazil.
  • Quines CB; Postgraduation Program in Biochemistry, Federal University of Pampa (UNIPAMPA), Uruguaiana, RS, Brazil.
  • Pinton S; Postgraduation Program in Biochemistry, Federal University of Pampa (UNIPAMPA), Uruguaiana, RS, Brazil.
Neural Regen Res ; 16(4): 783-789, 2021 Apr.
Article in En | MEDLINE | ID: mdl-33063743
Alzheimer's disease (AD) is a progressive brain disorder and complex mechanisms are involved in the physiopathology of AD. However, there is data suggesting that inflammation plays a role in its development and progression. Indeed, some non-steroidal anti-inflammatory drugs, such as meloxicam, which act by inhibiting cyclooxygenase-2 (COX-2) have been used as neuroprotective agents in different neurodegenerative disease models. The purpose of this study was to investigate the effects of co-nanoencapsulated curcumin and meloxicam in lipid core nanocapsules (LCN) on cognitive impairment induced by amyloid-beta peptide injection in mice. LCN were prepared by the nanoprecipitation method. Male Swiss mice received a single intracerebroventricular injection of amyloid-beta peptide aggregates (fragment 25-35, 3 nmol/3 µL) or vehicle and were subsequently treated with curcumin-loaded LCN (10 mg/kg) or meloxicam-loaded LCN (5 mg/kg) or meloxicam + curcumin-co-loaded LCN (5 and 10 mg/kg, respectively). Treatments were given on alternate days for 12 days (i.e., six doses, once every 48 hours, by intragastric gavage). Our data showed that amyloid-beta peptide infusion caused long-term memory deficits in the inhibitory avoidance and object recognition tests in mice. In the inhibitory avoidance test, both meloxicam and curcumin formulations (oil or co-loaded LCN) improved amyloid-beta-induced memory impairment in mice. However, only meloxicam and curcumin-co-loaded LCN attenuated non-aversive memory impairment in the object recognition test. Moreover, the beneficial effects of meloxicam and curcumin-co-loaded LCN could be explained by the anti-inflammatory properties of these drugs through cortical COX-2 downregulation. Our study suggests that the neuroprotective potential of meloxicam and curcumin co-nanoencapsulation is associated with cortical COX-2 modulation. This study was approved by the Committee on Care and Use of Experimental Animal Resources, the Federal University of Pampa, Brazil (approval No. 02-2015) on April 16, 2015.
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Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Neural Regen Res Year: 2021 Document type: Article Affiliation country: Brazil Country of publication: India

Full text: 1 Collection: 01-internacional Database: MEDLINE Language: En Journal: Neural Regen Res Year: 2021 Document type: Article Affiliation country: Brazil Country of publication: India