Methods for Crystallization and Structural Determination of M-T7 Protein from Myxoma Virus.

Gisriel, Christopher; Fromme, Petra; Martin-Garcia, Jose M

Gisriel, Christopher; Fromme, Petra; Martin-Garcia, Jose M.

Affiliation

Gisriel C; Department of Chemistry, Yale University, New Haven, CT, USA.
Fromme P; Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University, Tempe, AZ, USA.
Martin-Garcia JM; Center for Applied Structural Discovery, The Biodesign Institute, Arizona State University, Tempe, AZ, USA. Jose.Martingarcia@asu.edu.

Methods Mol Biol ; 2225: 125-162, 2021.

Article in En | MEDLINE | ID: mdl-33108661

Subject(s)

Crystallization/methods; Crystallography, X-Ray/methods; Myxoma virus/chemistry; Receptors, Interferon/ultrastructure; Viral Proteins/ultrastructure; Virulence Factors/genetics; X-Ray Diffraction/methods; Amino Acid Sequence; Cloning, Molecular; Cryoelectron Microscopy; Crystallography, X-Ray/instrumentation; Escherichia coli/genetics; Escherichia coli/metabolism; Gene Expression; Genetic Vectors/chemistry; Genetic Vectors/metabolism; Models, Molecular; Receptors, Interferon/chemistry; Receptors, Interferon/genetics; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/ultrastructure; Scattering, Small Angle; Sequence Alignment; Sequence Homology, Amino Acid; Viral Proteins/chemistry; Viral Proteins/genetics; Virulence Factors/metabolism; X-Ray Diffraction/instrumentation

Key words

Cryo-electron microscopy (cryo-EM); M-T7 protein; Micro-electron diffraction (Micro-ED); Myxoma virus; Small-angle X-ray scattering (SAXS); Structure determination; X-ray crystallography; X-ray free electron lasers (XFELs)

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Full text: 1 Collection: 01-internacional Database: MEDLINE Main subject: Viral Proteins / X-Ray Diffraction / Receptors, Interferon / Crystallography, X-Ray / Crystallization / Virulence Factors / Myxoma virus Type of study: Prognostic_studies Language: En Journal: Methods Mol Biol Journal subject: BIOLOGIA MOLECULAR Year: 2021 Document type: Article Affiliation country: United States Country of publication: United States

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